Genetic analyses of Schizosaccharomyces pombe dna2(+) reveal that Dna2 plays an essential role in Okazaki fragment metabolism

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dc.contributor.authorKang, Ho-Youngko
dc.contributor.authorChoi, Eunjooko
dc.contributor.authorBae, Sung-Hoko
dc.contributor.authorLee, Kyoung-Hwako
dc.contributor.authorGim, Byung-Sooko
dc.contributor.authorKim, Hee-Daiko
dc.contributor.authorPark, Chankyuko
dc.contributor.authorMacNeill, Stuart A.ko
dc.contributor.authorSeo, Yeon-Sooko
dc.date.accessioned2013-03-02T18:07:29Z-
dc.date.available2013-03-02T18:07:29Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2000-07-
dc.identifier.citationGENETICS, v.155, no.3, pp.1055 - 1067-
dc.identifier.issn0016-6731-
dc.identifier.urihttp://hdl.handle.net/10203/74838-
dc.description.abstractIn this report, we investigated the phenotypes caused by temperature-sensitive (ts) mutant alleles of dna(2+) of Schizosaccharomyces pombe, a homologue of DNA2 of budding yeast, in an attempt to further define its function in vivo with respect to lagging-strand synthesis during the S-phase of the cell cycle. At the restrictive temperature, dna2 (ts) cells arrested at late S-phase but were unaffected in bulk DNA synthesis. Moreover, they exhibited aberrant mitosis when combined xith checkpoint mutations, in keeping with a role for Dna2 in Okazaki fragment maturation. Similarly, spores in which dna2(+) was disrupted duplicated their DNA content during germination and also arrested at late S-phase. Inactivation of dna(2+) led to chromosome fragmentation strikingly similar to that seen when cdc17(+), the DNA ligase I gene, is inactivated The temperature-dependent lethality of dna2 (ts) mutants was suppressed by overexpression of genes encoding subunits of polymerase delta (cdc1(+) and cdc27(+)), DNA ligase I (cdc17(+)), and Fen-l (rad2(+)). Each of these gene products plays a role in the elongation or maturation of Okazaki fragments. Moreover, they all interacted with S. pombe Dna2 in a yeast two-hybrid assay, albeit to different extents. On the basis of these results, ive conclude that dna2(+) plays a direct role in the Okazaki fragment elongation and maturation. We propose that dna2(+) acts as a central protein to form a complex with other proteins required to coordinate the multienzyme process for Okazaki fragment elongation and maturation.-
dc.languageEnglish-
dc.publisherGENETICS-
dc.subjectCELL NUCLEAR ANTIGEN-
dc.subjectBASE EXCISION-REPAIR-
dc.subjectHIGH-EFFICIENCY TRANSFORMATION-
dc.subjectSINGLE-STRANDED-DNA-
dc.subjectSACCHAROMYCES-CEREVISIAE-
dc.subjectFISSION YEAST-
dc.subjectREPLICATION FORK-
dc.subjectPOLYMERASE-DELTA-
dc.subjectLIGASE-I-
dc.subjectENDONUCLEASE ACTIVITY-
dc.titleGenetic analyses of Schizosaccharomyces pombe dna2(+) reveal that Dna2 plays an essential role in Okazaki fragment metabolism-
dc.typeArticle-
dc.identifier.wosid000088056800007-
dc.identifier.scopusid2-s2.0-0033941545-
dc.type.rimsART-
dc.citation.volume155-
dc.citation.issue3-
dc.citation.beginningpage1055-
dc.citation.endingpage1067-
dc.citation.publicationnameGENETICS-
dc.contributor.localauthorPark, Chankyu-
dc.contributor.localauthorSeo, Yeon-Soo-
dc.contributor.nonIdAuthorKang, Ho-Young-
dc.contributor.nonIdAuthorChoi, Eunjoo-
dc.contributor.nonIdAuthorBae, Sung-Ho-
dc.contributor.nonIdAuthorLee, Kyoung-Hwa-
dc.contributor.nonIdAuthorGim, Byung-Soo-
dc.contributor.nonIdAuthorKim, Hee-Dai-
dc.contributor.nonIdAuthorMacNeill, Stuart A.-
dc.type.journalArticleArticle-
dc.subject.keywordPlusCELL NUCLEAR ANTIGEN-
dc.subject.keywordPlusBASE EXCISION-REPAIR-
dc.subject.keywordPlusHIGH-EFFICIENCY TRANSFORMATION-
dc.subject.keywordPlusSINGLE-STRANDED-DNA-
dc.subject.keywordPlusSACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusFISSION YEAST-
dc.subject.keywordPlusREPLICATION FORK-
dc.subject.keywordPlusPOLYMERASE-DELTA-
dc.subject.keywordPlusLIGASE-I-
dc.subject.keywordPlusENDONUCLEASE ACTIVITY-
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