DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Ho-Young | ko |
dc.contributor.author | Choi, Eunjoo | ko |
dc.contributor.author | Bae, Sung-Ho | ko |
dc.contributor.author | Lee, Kyoung-Hwa | ko |
dc.contributor.author | Gim, Byung-Soo | ko |
dc.contributor.author | Kim, Hee-Dai | ko |
dc.contributor.author | Park, Chankyu | ko |
dc.contributor.author | MacNeill, Stuart A. | ko |
dc.contributor.author | Seo, Yeon-Soo | ko |
dc.date.accessioned | 2013-03-02T18:07:29Z | - |
dc.date.available | 2013-03-02T18:07:29Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-07 | - |
dc.identifier.citation | GENETICS, v.155, no.3, pp.1055 - 1067 | - |
dc.identifier.issn | 0016-6731 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74838 | - |
dc.description.abstract | In this report, we investigated the phenotypes caused by temperature-sensitive (ts) mutant alleles of dna(2+) of Schizosaccharomyces pombe, a homologue of DNA2 of budding yeast, in an attempt to further define its function in vivo with respect to lagging-strand synthesis during the S-phase of the cell cycle. At the restrictive temperature, dna2 (ts) cells arrested at late S-phase but were unaffected in bulk DNA synthesis. Moreover, they exhibited aberrant mitosis when combined xith checkpoint mutations, in keeping with a role for Dna2 in Okazaki fragment maturation. Similarly, spores in which dna2(+) was disrupted duplicated their DNA content during germination and also arrested at late S-phase. Inactivation of dna(2+) led to chromosome fragmentation strikingly similar to that seen when cdc17(+), the DNA ligase I gene, is inactivated The temperature-dependent lethality of dna2 (ts) mutants was suppressed by overexpression of genes encoding subunits of polymerase delta (cdc1(+) and cdc27(+)), DNA ligase I (cdc17(+)), and Fen-l (rad2(+)). Each of these gene products plays a role in the elongation or maturation of Okazaki fragments. Moreover, they all interacted with S. pombe Dna2 in a yeast two-hybrid assay, albeit to different extents. On the basis of these results, ive conclude that dna2(+) plays a direct role in the Okazaki fragment elongation and maturation. We propose that dna2(+) acts as a central protein to form a complex with other proteins required to coordinate the multienzyme process for Okazaki fragment elongation and maturation. | - |
dc.language | English | - |
dc.publisher | GENETICS | - |
dc.subject | CELL NUCLEAR ANTIGEN | - |
dc.subject | BASE EXCISION-REPAIR | - |
dc.subject | HIGH-EFFICIENCY TRANSFORMATION | - |
dc.subject | SINGLE-STRANDED-DNA | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | FISSION YEAST | - |
dc.subject | REPLICATION FORK | - |
dc.subject | POLYMERASE-DELTA | - |
dc.subject | LIGASE-I | - |
dc.subject | ENDONUCLEASE ACTIVITY | - |
dc.title | Genetic analyses of Schizosaccharomyces pombe dna2(+) reveal that Dna2 plays an essential role in Okazaki fragment metabolism | - |
dc.type | Article | - |
dc.identifier.wosid | 000088056800007 | - |
dc.identifier.scopusid | 2-s2.0-0033941545 | - |
dc.type.rims | ART | - |
dc.citation.volume | 155 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 1055 | - |
dc.citation.endingpage | 1067 | - |
dc.citation.publicationname | GENETICS | - |
dc.contributor.localauthor | Park, Chankyu | - |
dc.contributor.localauthor | Seo, Yeon-Soo | - |
dc.contributor.nonIdAuthor | Kang, Ho-Young | - |
dc.contributor.nonIdAuthor | Choi, Eunjoo | - |
dc.contributor.nonIdAuthor | Bae, Sung-Ho | - |
dc.contributor.nonIdAuthor | Lee, Kyoung-Hwa | - |
dc.contributor.nonIdAuthor | Gim, Byung-Soo | - |
dc.contributor.nonIdAuthor | Kim, Hee-Dai | - |
dc.contributor.nonIdAuthor | MacNeill, Stuart A. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CELL NUCLEAR ANTIGEN | - |
dc.subject.keywordPlus | BASE EXCISION-REPAIR | - |
dc.subject.keywordPlus | HIGH-EFFICIENCY TRANSFORMATION | - |
dc.subject.keywordPlus | SINGLE-STRANDED-DNA | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | FISSION YEAST | - |
dc.subject.keywordPlus | REPLICATION FORK | - |
dc.subject.keywordPlus | POLYMERASE-DELTA | - |
dc.subject.keywordPlus | LIGASE-I | - |
dc.subject.keywordPlus | ENDONUCLEASE ACTIVITY | - |
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