DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, SJ | ko |
dc.contributor.author | Kim, DM | ko |
dc.contributor.author | Bae, KH | ko |
dc.contributor.author | Byun, SM | ko |
dc.contributor.author | Chung, Jae Hoon | ko |
dc.date.accessioned | 2013-02-27T20:59:39Z | - |
dc.date.available | 2013-02-27T20:59:39Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-02 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.66, no.2, pp.476 - 480 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/70810 | - |
dc.description.abstract | Staphylokinase (SAK), a polypeptide secreted by Staphylococcus aureus, is a plasminogen activator with a therapeutic potential in thrombosis diseases. A Bacillus subtilis strain which is multiply deficient in exoproteases was transformed by an expression plasmid carrying a promoter and a signal sequence of subtilisin fused in frame with the sak open reading frame. However, the amount of SAK secretion was marginal (45 mg/liter). In contrast, disruption of the wprA gene, which encodes a subtilisin-type protease, strongly promoted the production of SAK in the stationary phase (181 mg/liter). In addition, the extracellular stability of mature SAK. was dramatically enhanced. These data indicate a significant role of the wprA gene product in degrading foreign proteins, both during secretion and in the extracellular milieu. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | WALL-ASSOCIATED PROTEASE | - |
dc.subject | RECOMBINANT STAPHYLOKINASE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | THROMBOLYTIC THERAPY | - |
dc.subject | NUCLEOTIDE-SEQUENCE | - |
dc.subject | EXPRESSION | - |
dc.subject | STREPTOKINASE | - |
dc.subject | PURIFICATION | - |
dc.subject | PLASMINOGEN | - |
dc.subject | ARTERIAL | - |
dc.title | Enhancement of secretion and extracellular stability of staphylokinase in Bacillus subtilis by wprA gene disruption | - |
dc.type | Article | - |
dc.identifier.wosid | 000085125900003 | - |
dc.type.rims | ART | - |
dc.citation.volume | 66 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 476 | - |
dc.citation.endingpage | 480 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.identifier.doi | 10.1128/AEM.66.2.476-480.2000 | - |
dc.contributor.localauthor | Chung, Jae Hoon | - |
dc.contributor.nonIdAuthor | Lee, SJ | - |
dc.contributor.nonIdAuthor | Kim, DM | - |
dc.contributor.nonIdAuthor | Bae, KH | - |
dc.contributor.nonIdAuthor | Byun, SM | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | WALL-ASSOCIATED PROTEASE | - |
dc.subject.keywordPlus | RECOMBINANT STAPHYLOKINASE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | THROMBOLYTIC THERAPY | - |
dc.subject.keywordPlus | NUCLEOTIDE-SEQUENCE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | STREPTOKINASE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | PLASMINOGEN | - |
dc.subject.keywordPlus | ARTERIAL | - |
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