DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyoung Man | ko |
dc.date.accessioned | 2013-02-27T12:43:01Z | - |
dc.date.available | 2013-02-27T12:43:01Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996-01 | - |
dc.identifier.citation | FEMS MICROBIOLOGY LETTERS, v.135, no.1, pp.117 - 121 | - |
dc.identifier.issn | 0378-1097 | - |
dc.identifier.uri | http://hdl.handle.net/10203/68652 | - |
dc.description.abstract | A facultatively anaerobic bacterium producing an extracellular alkaline lipase was isolated from the soil collected near a sewage disposal plant in Korea and identified to be a strain of Proteus vulgaris. The molecular mass of the purified lipase K80 was estimated to be 31 kDa by SDS-PAGE. It was found to be an alkaline enzyme having maximum hydrolytic activity at pH 10, while fairly stable in a wide pH range from 5 to 11. The gene for lipase K80 was cloned in Escherichia coil. Sequence analysis showed an open reading frame of 861 bp coding for a polypeptide of 287 amino acid residues. The deduced amino acid sequence of the lipase gene had 46.3% identity to the lipase from Pseudomonas fragi. | - |
dc.language | English | - |
dc.publisher | Wiley-Blackwell | - |
dc.subject | PSEUDOMONAS-AERUGINOSA | - |
dc.subject | PURIFICATION | - |
dc.subject | CEPACIA | - |
dc.title | Characterization of an alkaline lipase from Proteus vulgaris K80 and the DNA sequence of the encoding gene | - |
dc.type | Article | - |
dc.identifier.wosid | A1996TP13800017 | - |
dc.identifier.scopusid | 2-s2.0-0030060987 | - |
dc.type.rims | ART | - |
dc.citation.volume | 135 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 117 | - |
dc.citation.endingpage | 121 | - |
dc.citation.publicationname | FEMS MICROBIOLOGY LETTERS | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | lipase K80 | - |
dc.subject.keywordAuthor | alkaline enzyme | - |
dc.subject.keywordAuthor | Proteus vulgaris | - |
dc.subject.keywordAuthor | enterobacterium | - |
dc.subject.keywordAuthor | lipase gene | - |
dc.subject.keywordPlus | PSEUDOMONAS-AERUGINOSA | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | CEPACIA | - |
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