DC Field | Value | Language |
---|---|---|
dc.contributor.author | 양진오 | ko |
dc.contributor.author | 정안식 | ko |
dc.contributor.author | 이성택 | ko |
dc.date.accessioned | 2013-02-27T08:08:40Z | - |
dc.date.available | 2013-02-27T08:08:40Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1987 | - |
dc.identifier.citation | 미생물학회지, v.25, no.4, pp.339 - 345 | - |
dc.identifier.issn | 4402-2413 | - |
dc.identifier.uri | http://hdl.handle.net/10203/67395 | - |
dc.description.abstract | A bacterium K-4-3, producing β-glucan hydrolyzing enzyme, was isolated from soil and identified to be Bacillus subtilis by its morpholohical and physiological characteristics. β-glucan was coloured using cibacron blue 3G-A and cross linded by the addition of 1, 4-butanedioldiglycidyl ether. This substrate was used for the isolation of β-glucanase producing microorganism. The β-glucan hydrolyzing enzyme actibity from isolated K-4-3 strain was also measured using the modified substrate. Bacillus subtilis K-4-3 produced the highest extracellular β-glucan hydrolyzing activity in the basal medium containing β-glucan as a carbon source, peptone and tryptone as a nitrogen source, and magnesium sulfate as an inorganic salt. The optimum temperature and initial pH for β-glucanase production by Bacillus subtilis K-4-3 were $37^{\circ}C$ and pH6. The highest enzyme activity was obtained at the culture age of 54 hrs with rotary shaking at $37^{\circ}C$. The crude enzyme showed the highest activity at pH 7.5-8.0 and 65℃. | - |
dc.language | Korean | - |
dc.publisher | 한국미생물학회 | - |
dc.title | 색소에 접합된 β-glucan을 이용한 β-glucan 분해효소 생산 균주의 분리 및 동정 | - |
dc.title.alternative | Isolation and identification of β-glucan degrading enzyme producing bacterium using coloured β-glucan | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 25 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 339 | - |
dc.citation.endingpage | 345 | - |
dc.citation.publicationname | 미생물학회지 | - |
dc.contributor.localauthor | 이성택 | - |
dc.contributor.nonIdAuthor | 양진오 | - |
dc.contributor.nonIdAuthor | 정안식 | - |
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