DC Field | Value | Language |
---|---|---|
dc.contributor.author | CHOI, PS | ko |
dc.contributor.author | SOH, WY | ko |
dc.contributor.author | KIM, YS | ko |
dc.contributor.author | Yoo, Ook-Joon | ko |
dc.contributor.author | LIU, JR | ko |
dc.date.accessioned | 2013-02-27T07:36:02Z | - |
dc.date.available | 2013-02-27T07:36:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1994-03 | - |
dc.identifier.citation | PLANT CELL REPORTS, v.13, no.6, pp.344 - 348 | - |
dc.identifier.issn | 0721-7714 | - |
dc.identifier.uri | http://hdl.handle.net/10203/67283 | - |
dc.description.abstract | Adventitious shoots formed on the Proximal cut edges of different cotyledonary explants of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai; cvs. Sweet Gem and Gold Medal] cultured on Murashige and Skoog's (MS) medium with 1 mgl-1 6-benzyladenine (BA). Light (16-h photoperiod, about 7 Wm-2 cool-white fluorescent lamps) was essential for shoot formation. To obtain transformed plants, cotyledonary explants of 'Sweet Gem' were cocultured with Agrobacterium tumefaciens LBA4404, a disarmed strain harboring a binary vector pBI121 carrying the CaMV 35S promoter-beta-glucuronidase (GUS) gene fusion used as a reporter gene and NOS promoter-neomycin phosphotransferase gene as a positive selection marker, for 48 h on MS medium with 1 mgl-1 BA and 200 muM beta-hydroxyacetosyringone. After 48 h of culture, explants were transferred to medium with 1 mgl-1 BA, 250 mgl-1 carbenicillin, and 100 mgl-1 kanamycin and cultured in the light. Adventitious shoots formed on the explants after 4 weeks of culture. When subjected to GUS histochemical assay, young leaves obtained from the shoots showed a positive response at a frequency of up to 16%. Preculturing cotyledonary explants on MS medium with 1 mgl-1 BA for 5 d enhanced the competence of the cells to be transformed by Agrobacterium Southern blot analysis confirmed that the GUS gene was incorporated into the genomic DNA of the GUS-positive regenerants. The transformed plants were grown to maturity. | - |
dc.language | English | - |
dc.publisher | SPRINGER VERLAG | - |
dc.subject | CITRULLUS-VULGARIS SCHRAD | - |
dc.subject | COTYLEDONS | - |
dc.title | GENETIC-TRANSFORMATION AND PLANT-REGENERATION OF WATERMELON USING AGROBACTERIUM-TUMEFACIENS | - |
dc.type | Article | - |
dc.identifier.wosid | A1994NE48300009 | - |
dc.type.rims | ART | - |
dc.citation.volume | 13 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 344 | - |
dc.citation.endingpage | 348 | - |
dc.citation.publicationname | PLANT CELL REPORTS | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.nonIdAuthor | CHOI, PS | - |
dc.contributor.nonIdAuthor | SOH, WY | - |
dc.contributor.nonIdAuthor | KIM, YS | - |
dc.contributor.nonIdAuthor | LIU, JR | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CITRULLUS-VULGARIS SCHRAD | - |
dc.subject.keywordPlus | COTYLEDONS | - |
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