대장균에서 많은 수의 플라스미드에 존재하는 rnpB 유전자 전사의 성장기의 따른 조절 작용Growth Phase - Dependent Control of Escherichia Coli rnpB Transcription in Multicopy Plasmids

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dc.contributor.author정영환ko
dc.contributor.author길민찬ko
dc.contributor.author전은순ko
dc.contributor.author은영아ko
dc.contributor.author이영훈ko
dc.contributor.author박충웅ko
dc.date.accessioned2013-02-25T23:17:56Z-
dc.date.available2013-02-25T23:17:56Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1993-
dc.identifier.citation한국유전학회지, v.15, no.2, pp.99 - 106-
dc.identifier.issn0254-5934-
dc.identifier.urihttp://hdl.handle.net/10203/65967-
dc.description.abstractThe Escherichia coli rnpB gene encodes M1 RNA, the catalytic component of RNase P which is a processing enzyme for tRNA maturation. Biosynthesis of M1 RNA is known to be stringently regulated. The rnpB transcription under different growth phases of E. coli was examined using the truncated rnpB in the multicopy plasmids which could generate the metabolically unstable M1 RNA transcript. Biosynthesis of this RNA was directly analyzed during the transition from the exponential phase of growth to the stationary phase. During the transition the rnpB transcription became repressed. The discriminator motif CGCC adjacent to the transcription start-point of the rnpB gene was altered to the TATT using site-directed mutagenesis. The modified promoter containing the mutated discriminator was at least partially resued from this growth phase-dependent repression of the rnpB transcription. The results clearly show that the discriminator motif play a key role in control of the rnpB transcription under different growth phases.-
dc.languageKorean-
dc.publisher한국유전학회-
dc.title대장균에서 많은 수의 플라스미드에 존재하는 rnpB 유전자 전사의 성장기의 따른 조절 작용-
dc.title.alternativeGrowth Phase - Dependent Control of Escherichia Coli rnpB Transcription in Multicopy Plasmids-
dc.typeArticle-
dc.type.rimsART-
dc.citation.volume15-
dc.citation.issue2-
dc.citation.beginningpage99-
dc.citation.endingpage106-
dc.citation.publicationname한국유전학회지-
dc.contributor.localauthor이영훈-
dc.contributor.nonIdAuthor정영환-
dc.contributor.nonIdAuthor길민찬-
dc.contributor.nonIdAuthor전은순-
dc.contributor.nonIdAuthor은영아-
dc.contributor.nonIdAuthor박충웅-
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CH-Journal Papers(저널논문)
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