핵자기 공명 분광학에 의한 Subitilisin Carlsberg 에 1 가 및 2 가 금속이온의 결합에 대한 연구Binding of Monovalent and Divalent Metal Ions to Subtilisin Carlsberg Probed by Proton Nuclear magnetic resonance Spectroscopy

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We have previously investigated the effects of removing bound Ca^(2+) ions and of adding KCl or CaCl₂ on the histidine C-2H resonances in subtilisin Carlsberg from Bacillus licheniformis by one-dimensional proton nuclear magnetic resonance spectroscopy [Korean Biochem. J. 22 (1989), 96-104]. As an extension of our previous work, effects of adding other monovalent (Li^+, Na^+, Cs^+) and divalent (Mg^(2+), Ba^(2+)) metal ions have been investigated to probe the nature of the metal ion binding sites. All monovalent ions show similar effects as do all divalent metal ions. However, the effects of monovalent and divalent ions are different from each other, particularly for the C-2H resonance of histidine 238. The results suggest that the low-affinity Ca^(2+) binding site in subtilisin Carlsberg and the adjacent monovalent cation binding site are not highly specific for any member of the divalent or monovalent metal ions, respectively, but they can differentiate between divalent and monovalent ions.
Publisher
생화학분자생물학회
Issue Date
1989
Language
English
Citation

KOREAN BIOCHEMICAL JOURNAL , v.22, no.4, pp.494 - 501

URI
http://hdl.handle.net/10203/65938
Appears in Collection
CH-Journal Papers(저널논문)
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