DC Field | Value | Language |
---|---|---|
dc.contributor.author | Guo, Wei | ko |
dc.contributor.author | Helfman, David M | ko |
dc.date.accessioned | 2013-02-25T22:08:13Z | - |
dc.date.available | 2013-02-25T22:08:13Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1993-10 | - |
dc.identifier.citation | NUCLEIC ACIDS RESEARCH, v.21, no.20, pp.4762 - 4768 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10203/65654 | - |
dc.description.abstract | We have been using the rat beta-tropomyosin (beta-TM) gene as a model system to study the mechanism of alternative splicing. The beta-TM gene spans 10 kb with 11 exons and encodes two distinct isoforms, namely skeletal muscle beta-TM and fibroblast TM-1. Exons 1 - 5, 8, and 9 are common to all mRNAs expressed from this gene. Exons 6 and 11 are used in fibroblasts, as well as in smooth muscle cells, whereas exons 7 and 1 0 are used exclusively in skeletal muscle cells. Our previous studies localized the critical elements for regulated alternative splicing to sequences within exon 7 and the adjacent upstream intron. We also demonstrated that these sequences function, in part, to regulate splice-site selection in vivo by interacting with cellular factors that block the use of the skeletal muscle exon in nonmuscle cells (1). Here we have further characterized the critical cis-acting elements involved in alternative splice site selection. Our data demonstrate that exon 7 and its flanking intron sequences are sufficient to regulate the suppression of exon 7 in nonmuscle cells when flanked by heterologous exons derived from adenovirus. We have also shown by both in vivo and in vitro assays that the blockage of exon 7 in nonmuscle cells is primarily at its 3'-splice site. A model is presented for regulated alternative splicing in both skeletal muscle and nonmuscle cells. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | PRE-MESSENGER-RNA | - |
dc.subject | BRANCH POINT LOCATION | - |
dc.subject | SEQUENCES | - |
dc.subject | INVITRO | - |
dc.subject | SELECTION | - |
dc.subject | TRANSCRIPTS | - |
dc.subject | INVIVO | - |
dc.subject | POLYADENYLATION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | BINDING | - |
dc.title | cis-elements involved in alternative splicing in the rat beta-tropomyosin gene: the 3-splice site of the skeletal muscle exon 7 is the major site of blockage in nonmuscle cells | - |
dc.type | Article | - |
dc.identifier.wosid | A1993MD03400017 | - |
dc.identifier.scopusid | 2-s2.0-0027508470 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 20 | - |
dc.citation.beginningpage | 4762 | - |
dc.citation.endingpage | 4768 | - |
dc.citation.publicationname | NUCLEIC ACIDS RESEARCH | - |
dc.identifier.doi | 10.1093/nar/21.20.4762 | - |
dc.contributor.localauthor | Helfman, David M | - |
dc.contributor.nonIdAuthor | Guo, Wei | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PRE-MESSENGER-RNA | - |
dc.subject.keywordPlus | BRANCH POINT LOCATION | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordPlus | INVITRO | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | TRANSCRIPTS | - |
dc.subject.keywordPlus | INVIVO | - |
dc.subject.keywordPlus | POLYADENYLATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | BINDING | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.