DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nam, Sang-Chul | ko |
dc.contributor.author | KANG, Changwon | ko |
dc.date.accessioned | 2008-07-17T02:24:22Z | - |
dc.date.available | 2008-07-17T02:24:22Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-11 | - |
dc.identifier.citation | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.38, no.6, pp.690 - 694 | - |
dc.identifier.issn | 1225-8687 | - |
dc.identifier.uri | http://hdl.handle.net/10203/6027 | - |
dc.description | http://www.jbmb.or.kr/view_article.php3?cont=jbmb&kid=173&mid=9&pid=9 | en |
dc.description.abstract | Transcription termination of the human mitochondrial genome requires specific binding to termination factor mTERF. In this study, mTERF was produced in E. coli and purified by two-step chromatography. mTERF-binding DNA sequences were isolated from a pool of randomized sequences by the repeated selection of bound sequences by gel-mobility shift assay and polymerase chain reaction. Sequencing and comparison of the 23 isolated clones revealed a 16-bp consensus sequence of 5'-GTGTGGCA GANCCNGG-3' in the light-strand (underlined residues were absolutely conserved), which nicely matched the genomic 13-bp terminator sequence 5'-TGGCAGAGCCCGG-3'. Moreover, mTERF binding assays of heteroduplex and single-stranded DNAs showed mTERF recognized the light strand in preference to the heavy strand. The preferential binding of mTERF with the light-strand may explain its distinct orientation-dependent termination activity. | - |
dc.description.sponsorship | the Korean Ministry of Science and Technology | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Korean Society for Biochemistry and Molecular Biology | - |
dc.title | DNA light-strand preferential recognition of human mitochondria transcription termination factor mTERF | - |
dc.type | Article | - |
dc.identifier.wosid | 000233627800009 | - |
dc.identifier.scopusid | 2-s2.0-32644433655 | - |
dc.type.rims | ART | - |
dc.citation.volume | 38 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 690 | - |
dc.citation.endingpage | 694 | - |
dc.citation.publicationname | JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | - |
dc.identifier.doi | 10.5483/bmbrep.2005.38.6.690 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | KANG, Changwon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | DNA-protein interaction | - |
dc.subject.keywordAuthor | DNA strand-specific binding | - |
dc.subject.keywordAuthor | human mitochondrial transcription | - |
dc.subject.keywordAuthor | MELAS mutation | - |
dc.subject.keywordAuthor | termination | - |
dc.subject.keywordAuthor | terminator | - |
dc.subject.keywordAuthor | termination factor mTERF | - |
dc.subject.keywordPlus | TRANSFER RNALEU(UUR) GENE | - |
dc.subject.keywordPlus | POINT MUTATION | - |
dc.subject.keywordPlus | MELAS SUBGROUP | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | ENCEPHALOMYOPATHIES | - |
dc.subject.keywordPlus | INVITRO | - |
dc.subject.keywordPlus | PROTEIN | - |
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