DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ravichandran, Naresh Kumar | ko |
dc.contributor.author | Kim, Hyemi | ko |
dc.contributor.author | Park, Joonha | ko |
dc.contributor.author | Hur, Hwan | ko |
dc.contributor.author | Kim, Jinsung | ko |
dc.contributor.author | Bae, Ji Yong | ko |
dc.contributor.author | Hyun, Sangwon | ko |
dc.contributor.author | Kim, I. Jong | ko |
dc.contributor.author | Kim, Dong Uk | ko |
dc.contributor.author | Lee, Sang-Chul | ko |
dc.contributor.author | Chang, Ki Soo | ko |
dc.contributor.author | Muniraj, Inbarasan | ko |
dc.contributor.author | Jeon, Jessie S. | ko |
dc.contributor.author | Nam, Ki-Hwan | ko |
dc.contributor.author | Lee, Kye-Sung | ko |
dc.date.accessioned | 2024-09-13T01:00:06Z | - |
dc.date.available | 2024-09-13T01:00:06Z | - |
dc.date.created | 2024-09-13 | - |
dc.date.issued | 2024-12 | - |
dc.identifier.citation | OPTICS AND LASER TECHNOLOGY, v.179 | - |
dc.identifier.issn | 0030-3992 | - |
dc.identifier.uri | http://hdl.handle.net/10203/322993 | - |
dc.description.abstract | Live-cell monitoring involves long-term observation and analysis of living cells in tissue cultures, which develop cells or tissues in an artificial environment and are essential for tissue engineering. Fluorescence microscopy (FM) is widely used as a monitoring tool owing to its powerful ability to visualize protein distribution within cells and tissues, thereby providing information on their functions in biological processes. To reduce the photobleaching effect, which is a major limiting factor in FM, FM is generally combined with other imaging modalities, such as phase contrast and differential interference contrast microscopy, which are nondestructive to fluorochromes, to selectively use fluorescence signals only in specific areas of interest within a sample. However, these methods are restricted to thin samples and cannot produce depth-resolved images. Here, we propose a method to determine three-dimensional (3D) positions in volumetric samples for application in high-resolution 3D fluorescence imaging using a multimode and multiscale imaging system that combines optical coherence microscopy (OCM) and line confocal FM (LC-FM). We also demonstrate the benefits of multimodal imaging in 3D cell culture monitoring. To evaluate the performance of the proposed system and method, we rapidly and accurately located the regions of interest in 3D tissue culture models, such as tumor spheroids and microvascular bed, and instantaneously acquired volumetric high-resolution fluorescence images. We also used an integrated system to monitor tumor spheroids mixed with extracellular matrix (ECM) over five days of the culture process, and the FM-OCM integrated technique successfully imaged the overall 3D structures, such as the distribution and boundaries of the spheroids and ECM as well as stained tumor cells. This complementary information is useful for understanding the relationship between cellular behaviors, such as the proliferation and migration of tumor cells, and microenvironments, such as the ECM. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Monitoring of three-dimensional live-cell cultures using a multimode, multiscale imaging system combining confocal fluorescence microscopy and optical coherence microscopy | - |
dc.type | Article | - |
dc.identifier.wosid | 001262765600001 | - |
dc.identifier.scopusid | 2-s2.0-85197069383 | - |
dc.type.rims | ART | - |
dc.citation.volume | 179 | - |
dc.citation.publicationname | OPTICS AND LASER TECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.optlastec.2024.111351 | - |
dc.contributor.localauthor | Jeon, Jessie S. | - |
dc.contributor.nonIdAuthor | Ravichandran, Naresh Kumar | - |
dc.contributor.nonIdAuthor | Kim, Hyemi | - |
dc.contributor.nonIdAuthor | Hur, Hwan | - |
dc.contributor.nonIdAuthor | Kim, Jinsung | - |
dc.contributor.nonIdAuthor | Bae, Ji Yong | - |
dc.contributor.nonIdAuthor | Hyun, Sangwon | - |
dc.contributor.nonIdAuthor | Kim, I. Jong | - |
dc.contributor.nonIdAuthor | Kim, Dong Uk | - |
dc.contributor.nonIdAuthor | Lee, Sang-Chul | - |
dc.contributor.nonIdAuthor | Chang, Ki Soo | - |
dc.contributor.nonIdAuthor | Muniraj, Inbarasan | - |
dc.contributor.nonIdAuthor | Nam, Ki-Hwan | - |
dc.contributor.nonIdAuthor | Lee, Kye-Sung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Optical coherence microscopy | - |
dc.subject.keywordAuthor | Extracellular matrix | - |
dc.subject.keywordAuthor | Tumor spheroid | - |
dc.subject.keywordAuthor | Vascular organoid | - |
dc.subject.keywordAuthor | Live-cell monitoring | - |
dc.subject.keywordAuthor | Fluorescence microscopy | - |
dc.subject.keywordPlus | TOMOGRAPHY | - |
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