High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy

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dc.contributor.authorLee, Moosungko
dc.contributor.authorJeong, Hyuntaeko
dc.contributor.authorLee, Chae Yeonko
dc.contributor.authorLee, Mahn Jaeko
dc.contributor.authorDelmo, Benedict Reveko
dc.contributor.authorHeo, Won Doko
dc.contributor.authorShin, Jennifer H.ko
dc.contributor.authorPark, Yongkeunko
dc.date.accessioned2024-09-05T01:00:16Z-
dc.date.available2024-09-05T01:00:16Z-
dc.date.created2024-08-29-
dc.date.issued2024-01-
dc.identifier.citationCOMMUNICATIONS BIOLOGY, v.7, no.1-
dc.identifier.urihttp://hdl.handle.net/10203/322617-
dc.description.abstractA critical requirement for studying cell mechanics is three-dimensional assessment of cellular shapes and forces with high spatiotemporal resolution. Traction force microscopy with fluorescence imaging enables the measurement of cellular forces, but it is limited by photobleaching and a slow acquisition speed. Here, we present refractive-index traction force microscopy (RI-TFM), which simultaneously quantifies the volumetric morphology and traction force of cells using a high-speed illumination scheme with 0.5-Hz temporal resolution. Without labelling, our method enables quantitative analyses of dry-mass distributions and shear (in-plane) and normal (out-of-plane) tractions of single cells on the extracellular matrix. When combined with a constrained total variation-based deconvolution algorithm, it provides 0.55-Pa shear and 1.59-Pa normal traction sensitivity for a 1-kPa hydrogel substrate. We demonstrate its utility by assessing the effects of compromised intracellular stress and capturing the rapid dynamics of cellular junction formation in the spatiotemporal changes in non-planar traction components. Label-free refractive-index traction force microscopy is established for cellular force probing quantification.-
dc.languageEnglish-
dc.publisherNATURE PORTFOLIO-
dc.titleHigh-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy-
dc.typeArticle-
dc.identifier.wosid001145732900001-
dc.identifier.scopusid2-s2.0-85182674848-
dc.type.rimsART-
dc.citation.volume7-
dc.citation.issue1-
dc.citation.publicationnameCOMMUNICATIONS BIOLOGY-
dc.identifier.doi10.1038/s42003-024-05788-4-
dc.contributor.localauthorHeo, Won Do-
dc.contributor.localauthorShin, Jennifer H.-
dc.contributor.localauthorPark, Yongkeun-
dc.contributor.nonIdAuthorJeong, Hyuntae-
dc.contributor.nonIdAuthorLee, Mahn Jae-
dc.contributor.nonIdAuthorDelmo, Benedict Reve-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusPHASE-
dc.subject.keywordPlusFLUORESCENCE-
dc.subject.keywordPlusILLUMINATION-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordPlusSIGNALS-
dc.subject.keywordPlusMOMENTS-
dc.subject.keywordPlusFIELDS-
dc.subject.keywordPlusT-CELLS-
dc.subject.keywordPlusDENDRITIC CELLS-
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