DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Moosung | ko |
dc.contributor.author | Jeong, Hyuntae | ko |
dc.contributor.author | Lee, Chae Yeon | ko |
dc.contributor.author | Lee, Mahn Jae | ko |
dc.contributor.author | Delmo, Benedict Reve | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.contributor.author | Shin, Jennifer H. | ko |
dc.contributor.author | Park, Yongkeun | ko |
dc.date.accessioned | 2024-09-05T01:00:16Z | - |
dc.date.available | 2024-09-05T01:00:16Z | - |
dc.date.created | 2024-08-29 | - |
dc.date.issued | 2024-01 | - |
dc.identifier.citation | COMMUNICATIONS BIOLOGY, v.7, no.1 | - |
dc.identifier.uri | http://hdl.handle.net/10203/322617 | - |
dc.description.abstract | A critical requirement for studying cell mechanics is three-dimensional assessment of cellular shapes and forces with high spatiotemporal resolution. Traction force microscopy with fluorescence imaging enables the measurement of cellular forces, but it is limited by photobleaching and a slow acquisition speed. Here, we present refractive-index traction force microscopy (RI-TFM), which simultaneously quantifies the volumetric morphology and traction force of cells using a high-speed illumination scheme with 0.5-Hz temporal resolution. Without labelling, our method enables quantitative analyses of dry-mass distributions and shear (in-plane) and normal (out-of-plane) tractions of single cells on the extracellular matrix. When combined with a constrained total variation-based deconvolution algorithm, it provides 0.55-Pa shear and 1.59-Pa normal traction sensitivity for a 1-kPa hydrogel substrate. We demonstrate its utility by assessing the effects of compromised intracellular stress and capturing the rapid dynamics of cellular junction formation in the spatiotemporal changes in non-planar traction components. Label-free refractive-index traction force microscopy is established for cellular force probing quantification. | - |
dc.language | English | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.title | High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy | - |
dc.type | Article | - |
dc.identifier.wosid | 001145732900001 | - |
dc.identifier.scopusid | 2-s2.0-85182674848 | - |
dc.type.rims | ART | - |
dc.citation.volume | 7 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | COMMUNICATIONS BIOLOGY | - |
dc.identifier.doi | 10.1038/s42003-024-05788-4 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.localauthor | Shin, Jennifer H. | - |
dc.contributor.localauthor | Park, Yongkeun | - |
dc.contributor.nonIdAuthor | Jeong, Hyuntae | - |
dc.contributor.nonIdAuthor | Lee, Mahn Jae | - |
dc.contributor.nonIdAuthor | Delmo, Benedict Reve | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PHASE | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | ILLUMINATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SIGNALS | - |
dc.subject.keywordPlus | MOMENTS | - |
dc.subject.keywordPlus | FIELDS | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
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