High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy

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A critical requirement for studying cell mechanics is three-dimensional assessment of cellular shapes and forces with high spatiotemporal resolution. Traction force microscopy with fluorescence imaging enables the measurement of cellular forces, but it is limited by photobleaching and a slow acquisition speed. Here, we present refractive-index traction force microscopy (RI-TFM), which simultaneously quantifies the volumetric morphology and traction force of cells using a high-speed illumination scheme with 0.5-Hz temporal resolution. Without labelling, our method enables quantitative analyses of dry-mass distributions and shear (in-plane) and normal (out-of-plane) tractions of single cells on the extracellular matrix. When combined with a constrained total variation-based deconvolution algorithm, it provides 0.55-Pa shear and 1.59-Pa normal traction sensitivity for a 1-kPa hydrogel substrate. We demonstrate its utility by assessing the effects of compromised intracellular stress and capturing the rapid dynamics of cellular junction formation in the spatiotemporal changes in non-planar traction components. Label-free refractive-index traction force microscopy is established for cellular force probing quantification.
Publisher
NATURE PORTFOLIO
Issue Date
2024-01
Language
English
Article Type
Article
Citation

COMMUNICATIONS BIOLOGY, v.7, no.1

DOI
10.1038/s42003-024-05788-4
URI
http://hdl.handle.net/10203/322617
Appears in Collection
BS-Journal Papers(저널논문)ME-Journal Papers(저널논문)PH-Journal Papers(저널논문)
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