DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 최명철 | - |
dc.contributor.author | Cho, Hasaeam | - |
dc.contributor.author | 조하새암 | - |
dc.date.accessioned | 2024-08-08T19:31:08Z | - |
dc.date.available | 2024-08-08T19:31:08Z | - |
dc.date.issued | 2024 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=1099237&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/322025 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 바이오및뇌공학과, 2024.2,[vii, 104 p. :] | - |
dc.description.abstract | Tau protein found in neurofibrillary tangle inside of a neuron, which is one of two conventional hallmarks of Alzheimer’s disease has been receiving large attention recently. Normal Tau is primarily known to bind to microtubules, an anionic cytoskeleton by electrostatic interaction. It is difficult to observe the conformation of Tau because it has almost no secondary or tertiary structure, so-called an intrinsically disordered protein (IDP) like a random coil. In addition, it is hard to predict the conformation of Tau, a polyampholyte that has both positive and negative charges. Therefore, the molecular structure and mechanism of Tau binding to microtubules are not yet elucidated. The main goal is to build a model of the association between Tau and microtubules and analyze the mechanism by monitoring structural changes of microtubules in the presence of Tau under ionic regulation. The hypothesis is that electrostatic interaction leads to the structural changes of Tau and microtubules. The Tau-microtubule bundles under controlled ionic strength and osmotic pressure were monitored and analyzed by small-angle X-ray scattering (SAXS), transmission electron microscopy (TEM), confocal laser scanning microscopy (CLSM), and western blot immunoassay techniques. Here, we suggest that the intermolecular structures of Tau bound to microtubules and changes in the physical property of Tau proteins under ionic regulation. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 타우▼a마이크로튜불▼a이온 세기▼a나노 구조▼a알츠하이머병▼a미접힘 단백질 | - |
dc.subject | Tau▼amicrotubule▼aionic strength▼anano-sturcutre▼aAlzheimer's disease▼aintrinsically disordered portein | - |
dc.title | Study of control in nano-structures of Tau and microtubule under ionic regulation | - |
dc.title.alternative | 조절된 이온 환경에서 타우와 마이크로튜불의 나노 구조 제어 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :바이오및뇌공학과, | - |
dc.contributor.alternativeauthor | Choi, Myung Chul | - |
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