Succinic acid-producing mutant microorganism into which high activity malate dehydrogenase is introduced, and method for preparing succinic acid by using same

Abstract

The present invention relates to: a succinic acid-producing mutant microorganism having improved activity of converting oxaloacetic acid to malic acid by the introduction of a gene encoding a malate dehydrogenase in which an amino acid residue interacting with a pyrophosphoric acid region of NADH through an amide functional group of a main chain is glutamine (Gln); and a method for preparing succinic acid by using same. The succinic acid-producing mutant microorganism according to the present invention has a remarkably increased activity of converting oxaloacetic acid to malic acid, and thus when the microorganism is cultured under a defined medium, high-concentration succinic acid can be produced with the highest succinic acid productivity from among that of mutant microorganisms reported thus far. In addition, by using an advanced fermentation technology, succinic acid can be produced with higher productivity and production concentrations.