DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kwak, Ju-Hyoun | - |
dc.contributor.advisor | 곽주현 | - |
dc.contributor.author | Hwang, Seong-Pil | - |
dc.contributor.author | 황성필 | - |
dc.date.accessioned | 2011-12-13T04:30:20Z | - |
dc.date.available | 2011-12-13T04:30:20Z | - |
dc.date.issued | 2005 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=244520&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/31647 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 화학과, 2005.2, [ iv, 76 p. ] | - |
dc.description.abstract | The underpotential deposition of silver and lead on Au(111) is performed sequentially to produce a thin composite layer. Ag was deposited underpotentially in 1 mM $Ag_2SO_4$ and 0.1 M $H_2SO_4$ at 0.400 V and 0.005 V vs Ag wire. Cyclic Voltammetry (CV) on this Ag/Au(111) electrode in $Pb^{2+}$ electrolyte with 0.1 M $HClO_4$ exhibited contributions of both Au and Ag substrates and in-situ STM images show unusual multilayer morphology of Pb upd on Ag/Au(111). CV demonstrated the stability and existence of Ag adlayer in $Pb^{2+}$ electrolyte. In the presence of sodium nitrate, this composite system shows the catalytic effect for nitrate reduction which is not observed in Au(111), Ag/Au(111), Pb/Au(111), Ag(111) and Pb/Ag(111) alone. All results demonstrate that the Ag-Pb/Au(111) composite system has the enhanced catalytic effect for nitrate reduction. We demonstrate the amplified detection of a target DNA based on the enzymatic deposition of silver. In this method, the target DNA and a biotinylated detection DNA probe hybridize to a capture DNA probe tethered onto a gold electrode. Neutravidin-conjugated alkaline phosphatase (Av-ALP) binds to the biotin of the detection probe on the electrode surface and converts the nonelectroactive substrate of the enzyme, p-aminophenyl phosphate (p-APP), into the reducing agent, p-aminophenol (p-AP). The latter, in turn, reduces metal ions in solutions leading to deposition of the metal onto the electrode surface and DNA backbone. This process, which we term biometallization, leads to a great enhancement in signal due to the accumulation of metallic silver by a catalytically generated enzyme product, and thus the electrochemical amplification of a biochemically amplified signal. The anodic stripping current of enzymatically deposited silver provides a measure of the extent of hybridization of the target oligomers. This biometallization process is highly sensitive, detecting as little as 100 aM (10 zmol) of DNA. We also success... | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Biosensor | - |
dc.subject | Electrocatalyst | - |
dc.subject | Metal deposition | - |
dc.subject | Electrochemistry | - |
dc.subject | DNA sensor | - |
dc.subject | DNA센서 | - |
dc.subject | 바이오센서 | - |
dc.subject | 전기화학촉매 | - |
dc.subject | 금속증착 | - |
dc.subject | 전기화학 | - |
dc.title | Electrocatalytic and bioelectroanalytical system based on metal deposition | - |
dc.title.alternative | 금속 증착에 기반한 전기화학 촉매와 생전기분석에 대한 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 244520/325007 | - |
dc.description.department | 한국과학기술원 : 화학과, | - |
dc.identifier.uid | 020015310 | - |
dc.contributor.localauthor | Kwak, Ju-Hyoun | - |
dc.contributor.localauthor | 곽주현 | - |
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