Electrocatalytic and bioelectroanalytical system based on metal deposition = 금속 증착에 기반한 전기화학 촉매와 생전기분석에 대한 연구

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dc.contributor.advisorKwak, Ju-Hyoun-
dc.contributor.advisor곽주현-
dc.contributor.authorHwang, Seong-Pil-
dc.contributor.author황성필-
dc.date.accessioned2011-12-13T04:30:20Z-
dc.date.available2011-12-13T04:30:20Z-
dc.date.issued2005-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=244520&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/31647-
dc.description학위논문(박사) - 한국과학기술원 : 화학과, 2005.2, [ iv, 76 p. ]-
dc.description.abstractThe underpotential deposition of silver and lead on Au(111) is performed sequentially to produce a thin composite layer. Ag was deposited underpotentially in 1 mM $Ag_2SO_4$ and 0.1 M $H_2SO_4$ at 0.400 V and 0.005 V vs Ag wire. Cyclic Voltammetry (CV) on this Ag/Au(111) electrode in $Pb^{2+}$ electrolyte with 0.1 M $HClO_4$ exhibited contributions of both Au and Ag substrates and in-situ STM images show unusual multilayer morphology of Pb upd on Ag/Au(111). CV demonstrated the stability and existence of Ag adlayer in $Pb^{2+}$ electrolyte. In the presence of sodium nitrate, this composite system shows the catalytic effect for nitrate reduction which is not observed in Au(111), Ag/Au(111), Pb/Au(111), Ag(111) and Pb/Ag(111) alone. All results demonstrate that the Ag-Pb/Au(111) composite system has the enhanced catalytic effect for nitrate reduction. We demonstrate the amplified detection of a target DNA based on the enzymatic deposition of silver. In this method, the target DNA and a biotinylated detection DNA probe hybridize to a capture DNA probe tethered onto a gold electrode. Neutravidin-conjugated alkaline phosphatase (Av-ALP) binds to the biotin of the detection probe on the electrode surface and converts the nonelectroactive substrate of the enzyme, p-aminophenyl phosphate (p-APP), into the reducing agent, p-aminophenol (p-AP). The latter, in turn, reduces metal ions in solutions leading to deposition of the metal onto the electrode surface and DNA backbone. This process, which we term biometallization, leads to a great enhancement in signal due to the accumulation of metallic silver by a catalytically generated enzyme product, and thus the electrochemical amplification of a biochemically amplified signal. The anodic stripping current of enzymatically deposited silver provides a measure of the extent of hybridization of the target oligomers. This biometallization process is highly sensitive, detecting as little as 100 aM (10 zmol) of DNA. We also success...eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectBiosensor-
dc.subjectElectrocatalyst-
dc.subjectMetal deposition-
dc.subjectElectrochemistry-
dc.subjectDNA sensor-
dc.subjectDNA센서-
dc.subject바이오센서-
dc.subject전기화학촉매-
dc.subject금속증착-
dc.subject전기화학-
dc.titleElectrocatalytic and bioelectroanalytical system based on metal deposition = 금속 증착에 기반한 전기화학 촉매와 생전기분석에 대한 연구-
dc.typeThesis(Ph.D)-
dc.identifier.CNRN244520/325007 -
dc.description.department한국과학기술원 : 화학과, -
dc.identifier.uid020015310-
dc.contributor.localauthorKwak, Ju-Hyoun-
dc.contributor.localauthor곽주현-
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