Electrocatalytic and bioelectroanalytical system based on metal deposition = 금속 증착에 기반한 전기화학 촉매와 생전기분석에 대한 연구

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The underpotential deposition of silver and lead on Au(111) is performed sequentially to produce a thin composite layer. Ag was deposited underpotentially in 1 mM $Ag_2SO_4$ and 0.1 M $H_2SO_4$ at 0.400 V and 0.005 V vs Ag wire. Cyclic Voltammetry (CV) on this Ag/Au(111) electrode in $Pb^{2+}$ electrolyte with 0.1 M $HClO_4$ exhibited contributions of both Au and Ag substrates and in-situ STM images show unusual multilayer morphology of Pb upd on Ag/Au(111). CV demonstrated the stability and existence of Ag adlayer in $Pb^{2+}$ electrolyte. In the presence of sodium nitrate, this composite system shows the catalytic effect for nitrate reduction which is not observed in Au(111), Ag/Au(111), Pb/Au(111), Ag(111) and Pb/Ag(111) alone. All results demonstrate that the Ag-Pb/Au(111) composite system has the enhanced catalytic effect for nitrate reduction. We demonstrate the amplified detection of a target DNA based on the enzymatic deposition of silver. In this method, the target DNA and a biotinylated detection DNA probe hybridize to a capture DNA probe tethered onto a gold electrode. Neutravidin-conjugated alkaline phosphatase (Av-ALP) binds to the biotin of the detection probe on the electrode surface and converts the nonelectroactive substrate of the enzyme, p-aminophenyl phosphate (p-APP), into the reducing agent, p-aminophenol (p-AP). The latter, in turn, reduces metal ions in solutions leading to deposition of the metal onto the electrode surface and DNA backbone. This process, which we term biometallization, leads to a great enhancement in signal due to the accumulation of metallic silver by a catalytically generated enzyme product, and thus the electrochemical amplification of a biochemically amplified signal. The anodic stripping current of enzymatically deposited silver provides a measure of the extent of hybridization of the target oligomers. This biometallization process is highly sensitive, detecting as little as 100 aM (10 zmol) of DNA. We also success...
Kwak, Ju-Hyounresearcher곽주현researcher
한국과학기술원 : 화학과,
Issue Date
244520/325007  / 020015310

학위논문(박사) - 한국과학기술원 : 화학과, 2005.2, [ iv, 76 p. ]


Biosensor; Electrocatalyst; Metal deposition; Electrochemistry; DNA sensor; DNA센서; 바이오센서; 전기화학촉매; 금속증착; 전기화학

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