Transcription termination and 3' Processing of M1 RNA, the RNA component of escherichia coli RNase P대장균 RNase P의 RNA 성분인 M1 RNA의 전사종결과 3' 가공과정

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M1 RNA is the RNA component of Escherichia coli RNase P, a tRNA-processing enzyme. When the rnpB gene encoding M1 RNA is transcribed, the primary M1 RNA transcript of 413 nucleotides (pM1 RNA) is produced and subsequently processed at the 3`` end to generate the mature M1 RNA of 377 nucleotides. However, the mechanism of the processing is relatively unexplored. The first aim of my study is to investigate features of pM1 RNA thought to be involved in the 3`` processing of M1 RNA. To examine the requirement of the M1 RNA sequence in the primary transcript for the processing reaction, first, I constructed plasmids containing the rnpB gene with a series of internal deletions in the M1 RNA sequence. The deletion plasmids containing at least both 56 nucleotides at the 5`` and 14 nucleotides at the 3`` in the M1 RNA sequence generated truncated transcripts processed normally at the 3`` end, suggesting that the intact M1 RNA sequence is not required for the 3`` processing. To study features of pM1 RNA thought to be involved in M1 RNA processing at 3`` end, the systematic mutations were introduced in sequence elements and secondary structures surrounding the processing site using p23 RNA, a truncated pM1 RNA transcribed from the internally deleted rnpB gene, as a model substrate. The processing of its mutant derivatives was analyzed in vivo and in vitro. Neither the alteration of two bases forming the processing site nor the disruption of secondary structures surrounding the site significantly affected the processing efficiency although the secondary structures were required for maintaining RNA stability. In contrast, the mutations at the rne-dependent site, GAUUU, immediately 3`` to the processing site inhibited the processing and the extent of the inhibition varied with the altered sequences. Although there is no simple relationship between the changes in the rne-dependent site and the inhibition of the processing, U nucleotides in the mutated sequences are preferred ...
Advisors
Lee, Young-Hoonresearcher이영훈researcher
Description
한국과학기술원 : 화학과,
Publisher
한국과학기술원
Issue Date
1997
Identifier
128462/325007 / 000945073
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 화학과, 1997.8, [ x, 129 p. ]

Keywords

RNase E; M1 RNA; Transcription termination; RNA processing; RNase P; RNase P; RNase E; M1 RNA; 전사종결; RNA 가공과정

URI
http://hdl.handle.net/10203/31463
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=128462&flag=dissertation
Appears in Collection
CH-Theses_Ph.D.(박사논문)
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