DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kang, Jin Young | - |
dc.contributor.advisor | 강진영 | - |
dc.contributor.author | Lee, Ji-Min | - |
dc.date.accessioned | 2023-06-26T19:32:33Z | - |
dc.date.available | 2023-06-26T19:32:33Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=997720&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/309728 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 화학과, 2022.2,[iv, 45 p :] | - |
dc.description.abstract | Riboswitch is a non-coding RNA element that modulates transcription or translation. It attracts much attention because it offers clues about the regulation of RNA polymerases and the development of antibiotic drugs. Pre$Q_1$-I riboswitch regulates transcription of operons related to queuosine biosynthesis. Previous structural studies show that it forms a pseudoknot fold by itself. However, the structure of the pre$Q_1$-I riboswitch bound to RNA polymerase is still unknown. Here, we report its structure determined using single-particle electron cryo-electron microscopy. Our cryo-EM structure reveals that a single-stranded RNA is located on the RNA exit channel and no nucleic acids protrude through the secondary channel. Furthermore, the polymerase in the riboswitch complex shows a swiveling movement related to the elongation complex's pausing mechanism. These results are distinct from those of hairpin pause or the backtracking pause. Our structural study will help a deeper understanding of prokaryotic gene regulation by riboswitches. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.title | Structural basis of que pause in the pre$Q_1$-I riboswitch-bound RNA polymerase elongation complex | - |
dc.title.alternative | pre$Q_1$-I 리보소의치가 결합한 RNA 중합효소 복합체에서의 que pause 구조적 규명 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :화학과, | - |
dc.contributor.alternativeauthor | 이지민 | - |
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