The (EDTA)4 - organic substituents were supplemented with alkaline silicates electrolyte, however in varying concentration to explore the microstructure, chemical composition and subsequent effects on the biodegradation of the PEO coated Mg AZ91 alloy. From surface analysis-, carried out through SEM, samples with zero-(EDTA)4 - were found-, lacking pancake structural features; however, supplementing electrolyte with (EDTA)4 -, bulky and layered pancakes were obtained with minimum cracks and minimum porosity for EDT-1. From the phase and inner layer analysis, it was found that EDTA can significantly alter the surface layers composition and could act as a passivation agent-; however, individual polycrystalline phases were not found to form. The hardness value obtained for EDT-1 was similar to 909.69 HV, the highest value found here which occurs with the shallowest indents found in this study. In-vitro degradation analysis was carried out using potentiodynamic polarization at room temperature, cyclic polarization at RT and high fever conditions (HFT similar to 40 degrees C). The corrosion potential for coated AZ91 was obtained as a positive value of similar to 0.1 V, for the first time in the PEO research; however, the results were not supported by the cyclic polarization tests at room temperature and at HFT. The post corrosion microstructural analysis confirmed the effective role of EDTA up to 1.0 g center dot L -1 and revealed the formation of thin films during the forward polarization at HFT.(c) 2022 Chongqing University. Publishing services provided by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) Peer review under responsibility of Chongqing University