Predicting the effects of cultivation condition on gene regulation in Escherichia coli by using deep learning

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 111
  • Download : 0
Cell's physiology is affected by cultivation conditions at varying degrees, including carbon sources and inorganic nutrients in growth medium, and the presence or absence of aeration. When examining the effects of cultivation conditions on the cell, the cell's transcriptional response is often examined first among other phenotypes (e.g., proteome and metabolome). In this regard, we developed DeepMGR, a deep learning model that predicts the effects of culture media on gene regulation in Escherichia coli. DeepMGR specifically classifies the direction of gene regulation (i.e., upregulation, no regulation, or downregulation) for an input gene in comparison with M9 minimal medium with glucose as a control condition. For this classification task, DeepMGR uses a feedforward neural network to process: i) DNA sequence of a target gene, ii) presence or absence of aeration and trace elements, and iii) concentration and structural information (SMILES) of up to ten nutrients. The complete DeepMGR showed accuracy of 0.867 and F1 score of 0.703 for a test set from the gold standard dataset. DeepMGR was further subjected to simulation studies for validation where regulation directions for groups of homologous genes were predicted, and the DeepMGR results were compared with the literature with focus on carbon sources that upregulate specific genes. DeepMGR will be useful for designing experiments to understand gene regulations, especially in the context of metabolic engineering. (c) 2023 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Publisher
ELSEVIER
Issue Date
2023-04
Language
English
Article Type
Article
Citation

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, v.21, pp.2613 - 2620

ISSN
2001-0370
DOI
10.1016/j.csbj.2023.04.010
URI
http://hdl.handle.net/10203/306901
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0