Simultaneous expansion microscopy imaging of proteins and mRNAs via dual-ExM

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<jats:title>Abstract</jats:title><jats:p>Simultaneous nanoscale imaging of mRNAs and proteins of the same specimen can provide better information on the translational regulation, molecular trafficking, and molecular interaction of both normal and diseased biological systems. Expansion microscopy (ExM) is an attractive option to achieve such imaging; however, simultaneous ExM imaging of proteins and mRNAs has not been demonstrated. Here, a technique for simultaneous ExM imaging of proteins and mRNAs in cultured cells and tissue slices, which we termed dual-expansion microscopy (dual-ExM), is demonstrated. First, we verified a protocol for the simultaneous labeling of proteins and mRNAs. Second, we combined the simultaneous labeling protocol with ExM to enable the simultaneous ExM imaging of proteins and mRNAs in cultured cells and mouse brain slices and quantitatively study the degree of signal retention after expansion. After expansion, both proteins and mRNAs can be visualized with a resolution beyond the diffraction limit of light in three dimensions. Dual-ExM is a versatile tool to study complex biological systems, such as the brain or tumor microenvironments, at a nanoscale resolution.</jats:p>
Publisher
NATURE RESEARCH
Issue Date
2022-02
Language
English
Article Type
Article
Citation

SCIENTIFIC REPORTS, v.12, no.1

ISSN
2045-2322
DOI
10.1038/s41598-022-06903-3
URI
http://hdl.handle.net/10203/294756
Appears in Collection
MS-Journal Papers(저널논문)
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