The hardening behavior of AISI 304 steel is investigated at various strain rates, from the quasi-static state to ultra-high strain rates, because it is necessary for numerical simulation of high-speed deformation problems. This kind of testing at a wide range of strain rates has not been yet reported in the literature although it is indispensable for accurate numerical analyses where deformation takes place with a wide spectrum of strain rates. AISI 304 steel is a kind of austenitic stainless steel used in various engineering fields, which does not harden by heat treatment, but by cold working such as shot peening. In order to obtain hardening properties at each strain rate, tensile tests were carried out using a universal testing machine of the INSTRON 5583 for the quasi-static state, a high speed material testing machine of a servo-hydraulic type for intermediate strain rates, and a tensile split-Hopkinson bar for high strain rates with a digital image correlation method. The hardening properties at the ultra-high strain-rate region were obtained from Taylor impact test results by calibration with an experimental-numerical hybrid inverse optimization for reliable extrapolation results. Finally, the hardening flow stress curves were obtained at various strain rates from the quasi-static state to ultra-high strain rates by interpolating the data with the extended Lim-Huh model. The result shows that the yield stress of 759 MPa at the quasi-static state increased to 1429 MPa at a strain rate of 106 s-1, which is about 1.9 times of the quasi-static yield stress. As a demonstration example, the dynamic hardening properties obtained were applied to a shot peening simulation that required hardening curves at a wide range of strain rates from the static state to 106 s-1. The simulation result with the dynamic hardening properties is compared to that with the quasi-static properties. The comparison shows a notable difference in the maximum compressive residual stress by 32%, demonstrating that it is important to consider the dynamic hardening properties in such high strain rate simulation as shot peening for accurate simulation results.