Bioremediation and monitoring of petroleum hydrocarbon contaminated site by molecular biology tools = 분자생물학적 방법을 이용한 석유계 탄화수소 오염 현장의 생물학적 정화 및 모니터링

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Biological methods for restoration suggest an improved substitute for ineffective and costly phsico.chemical remediation methods. However, only a small fraction of the total microbial ecosystem has been harnessed for this purpose. Exploring and exploiting unrevealed microbial community associated with the degradation of xenobiotics can provide insight into the development ‘Biocatalysts’ for environmental restoration. In this study, culture independent approaches such as analysis of microbial diversity and ecology, and detection of catabolic genes were used to improve the process of efficacy determination and implementation of microbial bioremediation strategies in petroleum hydrocarbon contaminated site (soil and groundwater). For remediation of diesel contaminated soil, intrinsic biodegradation potential was estimated by analysis of microbial communities and diesel-assimilating bacteria were isolated. Comparative bioremediation showed that despite the existence of native diesel assimilating bacteria, natural attenuation and biostimulation were not effective in diesel biodegradation and enhanced biodegradation efficiencies were observed in bioaugmentation with enriched diesel-assimilating strains. From this result, bioaugmentation with an indigenous diesel assimilating bacteria (PO1, identified as $\It{Rhodococcus}$ sp.) and two exogenous strains was performed and microbial activity and community structure were monitored during bioaugmentation. It was demonstrated that the bioaugmentation enhanced degradation efficiency and the greatest degradation of TPH (total petroleum hydrocarbon) was achieved after six weeks of the experiment (55.9%). The microbial number and enzyme activity were higher in bioaugmentation than in control (natural attenuation) and the results were correlated with TPH degradation, indicating the positive influence of treatment. However, increase in total bacterial number and microbial activity did not correlated with TPH degradation. Thus,...
Yang, Ji-Wonresearcher양지원researcher
한국과학기술원 : 생명화학공학과,
Issue Date
455368/325007  / 020055017

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2010.08, [ ⅸ, 97 p. ]


DGGE; Petroleum; Biomonitoring; Bioremediation; Microbial community; 미생물 군집; DGGE; 석유계 탄화수소; 모니터링; 생물학적 복원

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