Gene-expression analyses of acidic pH shock effects on actinorhodin production in Streptomyces coelicolor A3(2) = Acidic pH shock 이 Streptomyces coelicolor에 의한 actinorhodin 생산에 미치는 영향에 대한 발현학적 해석

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Actinorhodin production is markedly enhanced when an acidic pH shock is applied to a surface-grown culture of Streptomyces coelicolor A3(2). For an in-depth study of this phenomenon, transcriptional analyses by DNA microarras, and RT-PCR and proteomic analysis methods were performed. Investigated were expression levels of the regulators and enzymes responsible for signal transduction and actinorhodin biosynthesis, and enzymes involved in some major metabolic pathways such as glycolysis, the tricarboxylic acid cycle, fatty acid metabolism, and the pentose phosphate pathway (PPP). In addition, the effects of pH shock on the stress response related genes to investigate the relationship between acidic pH shock and other shocks, through transcriptional and proteomic analysis also. The regulators PkaG, AfsR, AfsS and/or another unidentified regulator and ActII-ORF4, in sequence, were observed to be activated by pH shock. In addition, a number of genes associated with actinorhodin production and secretion, and the major central metabolic pathways investigated were observed to be up-regulated with pH shock. Fatty acid degradation was particularly promoted by pH shock, while fatty acid biosynthesis, consuming acetyl-CoA was suppressed; it is envisaged that this enriches the precursor pool (acetyl-CoA) and building blocks for actinorhodin biosynthesis. Furthermore, glucose 6-phosphate dehydrogenases (zwf and zwf2), key enzymes to initiate the PPP, were highly activated by pH shock, enriching the NADPH pool for biosynthesis in general. It is deduced that these metabolic changes caused by pH shock have positively contributed to the stimulation of actinorhodin biosynthesis in a concerted manner. A number of shock related proteins were observed to be induced by acidic pH shock. Well known sigma factors of sigH (by heat shock), sigR (by oxidative stress), and sigB (by osmotic stress) which play a major role in the secondary metabolism, were all strongly upregulated in th...
Chang, Yong-Keunresearcher장용근researcher
한국과학기술원 : 생명화학공학과,
Issue Date
263444/325007  / 020015067

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2007.2, [ x, 130 p. ]


actinorhodin production; S. coelicolor; pH shock; expression analysis; 발현분석; 액티노로딘 생산; 스트렙토마이시스 실리컬러; 산충격

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