Engineering of Klebsiella oxytoca for the Production of 2,3-Butanediol from High Concentration of Xylose

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Klebsiella oxytoca is widely used for the biological production of 2,3-butanediol (2,3-BDO), a promising platform chemical with a broad range of applications. Here, to improve cell growth and production of 2,3-BDO under high concentration of xylose (100 g/L), we engineered K. oxytoca using an adaptive laboratory evolution and a biosensor-derived high throughput screening strategy. First, we developed a XylR-dependent xylose biosensor for the detection of intracellular xylose, and K. oxytoca containing the xylose biosensor was used for adaptive laboratory evolution in 100 g/L xylose. Cells were isolated by FACS screening, and the isolated strain (KO8S16) showed much improved cell growth with high xylose consumption rate (1.35 g/L/h) and 2,3-BDO productivity (0.53 g/L/h) compared with the wild-type strain. Through whole genome resequencing, it was revealed that a mutation in OmpR (a response regulator of osmotic stress) allowed to withstand high concentrations of xylose. Finally, fed-batch cultivation was performed by feeding high concentration of xylose, and K. oxytoca successfully produced 2,3-BDO at a concentration as high as 57.5 g/L by consuming 238.13 g/L xylose in 47 h.
Publisher
AMER CHEMICAL SOC
Issue Date
2021-11
Language
English
Article Type
Article
Citation

ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v.9, no.43, pp.14395 - 14404

ISSN
2168-0485
DOI
10.1021/acssuschemeng.1c04118
URI
http://hdl.handle.net/10203/289367
Appears in Collection
CBE-Journal Papers(저널논문)
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