DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, Ae Jin | ko |
dc.contributor.author | Jeong, Byeong-ryool | ko |
dc.contributor.author | Kang, Nam Kyu | ko |
dc.contributor.author | Jeon, Seungjib | ko |
dc.contributor.author | Sohn, Min Gi | ko |
dc.contributor.author | Yun, Hyo Jin | ko |
dc.contributor.author | Lim, Jong Min | ko |
dc.contributor.author | Jeong, Seok Won | ko |
dc.contributor.author | Park, Youn-Il | ko |
dc.contributor.author | Jeong, Won Joong | ko |
dc.contributor.author | Park, Sunghoon | ko |
dc.contributor.author | Chang, Yong Keun | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2021-09-28T01:10:26Z | - |
dc.date.available | 2021-09-28T01:10:26Z | - |
dc.date.created | 2021-09-28 | - |
dc.date.created | 2021-09-28 | - |
dc.date.created | 2021-09-28 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.citation | BIORESOURCE TECHNOLOGY, v.340 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | http://hdl.handle.net/10203/287891 | - |
dc.description.abstract | Transgene expression in microalgae can be hampered by transgene silencing and unstable expression due to position effects. To overcome this, "safe harboring" transgene expression system was established for Nanno-chloropsis. Initially, transformants were obtained expressing a sfGFP reporter, followed by screening for high expression of sfGFP with fluorescence-activated cell sorter (FACS). 'T1' transcriptional hotspot was identified from a mutant showing best expression of sfGFP, but did not affect growth or lipid contents. By using a Cas9 editor strain, FAD12 gene, encoding Delta 12-fatty acid desaturase (FAD12), was successfully knocked-in at the T1 locus, resulting in significantly higher expression of FAD12 than those of random integration. Importantly, the "safe harbored" FAD12 transformants showed four-fold higher production of linoleic acid (LA), the product of FAD12, leading to 1.5-fold increase in eicosapentaenoic acid (EPA). This safe harboring principle provide excellent proof of the concept for successful genetic/metabolic engineering of microalgae and other organisms. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Safe-Harboring based novel genetic toolkit for Nannochloropsis salina CCMP1776: Efficient overexpression of transgene via CRISPR/Cas9-Mediated Knock-in at the transcriptional hotspot | - |
dc.type | Article | - |
dc.identifier.wosid | 000696313700004 | - |
dc.identifier.scopusid | 2-s2.0-85112485517 | - |
dc.type.rims | ART | - |
dc.citation.volume | 340 | - |
dc.citation.publicationname | BIORESOURCE TECHNOLOGY | - |
dc.identifier.doi | 10.1016/j.biortech.2021.125676 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Jeong, Byeong-ryool | - |
dc.contributor.localauthor | Chang, Yong Keun | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Sohn, Min Gi | - |
dc.contributor.nonIdAuthor | Yun, Hyo Jin | - |
dc.contributor.nonIdAuthor | Lim, Jong Min | - |
dc.contributor.nonIdAuthor | Jeong, Seok Won | - |
dc.contributor.nonIdAuthor | Park, Youn-Il | - |
dc.contributor.nonIdAuthor | Jeong, Won Joong | - |
dc.contributor.nonIdAuthor | Park, Sunghoon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CRISPR/Cas9 | - |
dc.subject.keywordAuthor | Safe harboring | - |
dc.subject.keywordAuthor | Nannochloropsis salina | - |
dc.subject.keywordAuthor | Fatty acid desaturase | - |
dc.subject.keywordAuthor | Knock-in via NHEJ | - |
dc.subject.keywordPlus | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | ACYLTRANSFERASES | - |
dc.subject.keywordPlus | STRAINS | - |
dc.subject.keywordPlus | PROTEIN | - |
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