Enhanced production of neoagarobiose from agar with Corynebacterium glutamicum producing exo-type and endo-type beta-agarases

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 21
  • Download : 0
Neoagarobiose (NA2) derived from agar marine biomass is a rare reagent that acts as an anti-melanogenesis reagent and moisturizer. Here, for the economical manufacturing of NA2, we developed the co-secretory production system of endo-type beta-agarases (DagA) and exo-type beta-agarases (EXB3) in Corynebacterium glutamicum. For this purpose, we first developed a secretory system of DagA via Tat pathway. To improve the secretion efficiency, we coexpressed two Tat pathway components (TatA and TatC), and to improve the purity of secreted DagA in the culture supernatant, two endogenous protein genes (Cg2052 and Cg1514) were removed. Using the engineered strain (C. glutamicum SP002), we confirmed that DagA as high as 1.53 g l(-1) was successfully produced in the culture media with high purity (72.7% in the supernatant protein fraction). Next, we constructed the expression system (pHCP-CgR-DagA-EXB3) for the simultaneous secretion of EXB3 via Sec-pathway together with DagA, and it was clearly confirmed that DagA and EXB3 were successfully secreted as high as 54% and 24.5%, respectively. Finally, using culture medium containing DagA and EXB3, we successfully demonstrated the conversion of high-concentration agar (40 g l(-1)) into NA2 via a two-stage hydrolysis process.
Publisher
WILEY
Issue Date
2021-09
Language
English
Article Type
Article
Citation

MICROBIAL BIOTECHNOLOGY, v.14, no.5, pp.2164 - 2175

ISSN
1751-7915
DOI
10.1111/1751-7915.13899
URI
http://hdl.handle.net/10203/287881
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0