Stepwise transmigration of T- and B cells through a perivascular channel in high endothelial venules

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High endothelial venules (HEVs) effectively recruit circulating lymphocytes from the blood to lymph nodes. HEVs have endothelial cells (ECs) and perivascular sheaths consisting of fibroblastic reticular cells (FRCS). Yet, post-luminal lymphocyte migration steps are not well elucidated. Herein, we performed intravital imaging to investigate post-luminal T- and B-cell migration in popliteal lymph node, consisting of trans-EC migration, crawling in the perivascular channel (a narrow space between ECs and FRCS) and trans-FRC migration. The post-luminal migration of T cells occurred in a PNAd-dependent manner. Remarkably, we found hot spots for the trans-EC and trans-FRC migration of T- and B cells. Interestingly, T- and B cells preferentially shared trans-FRC migration hot spots but not trans-EC migration hot spots. Furthermore, the trans-FRC T-cell migration was confined to fewer sites than trans-EC T-cell migration, and trans-FRC migration of T- and B cells preferentially occurred at FRCS covered by CD11c+ dendritic cells in HEVs. These results suggest that HEV ECs and FRCS with perivascular DCs delicately regulate T- and B-cell entry into peripheral lymph nodes.
Publisher
LIFE SCIENCE ALLIANCE LLC
Issue Date
2021-08
Language
English
Article Type
Article
Citation

LIFE SCIENCE ALLIANCE, v.4, no.8

ISSN
2575-1077
DOI
10.26508/lsa.202101086
URI
http://hdl.handle.net/10203/287869
Appears in Collection
MSE-Journal Papers(저널논문)
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