DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Yunho | ko |
dc.contributor.author | Song, Younseong | ko |
dc.contributor.author | Kim, Yong Tae | ko |
dc.contributor.author | Kim, Hogi | ko |
dc.contributor.author | Park, Yoo Min | ko |
dc.contributor.author | Lee, Seok Jae | ko |
dc.contributor.author | Im, Sung Gap | ko |
dc.contributor.author | Lee, Kyoung G. | ko |
dc.date.accessioned | 2021-08-09T06:10:08Z | - |
dc.date.available | 2021-08-09T06:10:08Z | - |
dc.date.created | 2021-08-09 | - |
dc.date.created | 2021-08-09 | - |
dc.date.created | 2021-08-09 | - |
dc.date.issued | 2021-10 | - |
dc.identifier.citation | SENSORS AND ACTUATORS B-CHEMICAL, v.344, pp.130307 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/10203/287074 | - |
dc.description.abstract | Point-of-care testing (POCT) is highly desirable for early detection of infectious diseases; however, current molecular diagnostics require a series of sophisticated facilities and instruments, which critically limits its direct application to POCT. From this perspective, chip-scale microfluidic technologies have been widely investigated to provide rapid, low-cost POCT diagnostics. Despite the development of small-footprint portable microfluidic chips for molecular diagnostics, the integration of functional steps into POCT is still a crucial, unsolved issue to be addressed. Herein, we suggest an all-in-one pumpless portable genetic analysis microsystem for molecular diagnostics with facile and portable operation by suggesting three main functions: (i) a finger-powered actuator for exquisite fluid control, (ii) a poly(dimethylaminomethyl styrene)-based polymeric surface for efficient nucleic acid harvest and extraction, and (iii) loop-mediated isothermal amplification-based amplification and colorimetric detection. The developed system can be stored at room temperature, shows a wide detection range from 1 x 103 to 1 x 107 cells, and enables naked-eye detection with a total detection time of less than 80 min. This allin-one pumpless portable genetic analysis microsystem can be utilized as a simple and accurate platform for POCT diagnostics. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.title | All-in-one pumpless portable genetic analysis microsystem for rapid naked-eye detection | - |
dc.type | Article | - |
dc.identifier.wosid | 000678140800003 | - |
dc.identifier.scopusid | 2-s2.0-85108104480 | - |
dc.type.rims | ART | - |
dc.citation.volume | 344 | - |
dc.citation.beginningpage | 130307 | - |
dc.citation.publicationname | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.identifier.doi | 10.1016/j.snb.2021.130307 | - |
dc.contributor.localauthor | Im, Sung Gap | - |
dc.contributor.nonIdAuthor | Kim, Yong Tae | - |
dc.contributor.nonIdAuthor | Park, Yoo Min | - |
dc.contributor.nonIdAuthor | Lee, Seok Jae | - |
dc.contributor.nonIdAuthor | Lee, Kyoung G. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Point-of-care testing | - |
dc.subject.keywordAuthor | Molecular diagnostics | - |
dc.subject.keywordAuthor | Pumpless microchip | - |
dc.subject.keywordAuthor | Foodborne pathogens | - |
dc.subject.keywordAuthor | Initiated chemical vapor deposition | - |
dc.subject.keywordPlus | ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | TESTING SYSTEM | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | DEVICE | - |
dc.subject.keywordPlus | PAPER | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
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