All-in-one pumpless portable genetic analysis microsystem for rapid naked-eye detection

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dc.contributor.authorChoi, Yunhoko
dc.contributor.authorSong, Younseongko
dc.contributor.authorKim, Yong Taeko
dc.contributor.authorKim, Hogiko
dc.contributor.authorPark, Yoo Minko
dc.contributor.authorLee, Seok Jaeko
dc.contributor.authorIm, Sung Gapko
dc.contributor.authorLee, Kyoung G.ko
dc.date.accessioned2021-08-09T06:10:08Z-
dc.date.available2021-08-09T06:10:08Z-
dc.date.created2021-08-09-
dc.date.created2021-08-09-
dc.date.created2021-08-09-
dc.date.issued2021-10-
dc.identifier.citationSENSORS AND ACTUATORS B-CHEMICAL, v.344, pp.130307-
dc.identifier.issn0925-4005-
dc.identifier.urihttp://hdl.handle.net/10203/287074-
dc.description.abstractPoint-of-care testing (POCT) is highly desirable for early detection of infectious diseases; however, current molecular diagnostics require a series of sophisticated facilities and instruments, which critically limits its direct application to POCT. From this perspective, chip-scale microfluidic technologies have been widely investigated to provide rapid, low-cost POCT diagnostics. Despite the development of small-footprint portable microfluidic chips for molecular diagnostics, the integration of functional steps into POCT is still a crucial, unsolved issue to be addressed. Herein, we suggest an all-in-one pumpless portable genetic analysis microsystem for molecular diagnostics with facile and portable operation by suggesting three main functions: (i) a finger-powered actuator for exquisite fluid control, (ii) a poly(dimethylaminomethyl styrene)-based polymeric surface for efficient nucleic acid harvest and extraction, and (iii) loop-mediated isothermal amplification-based amplification and colorimetric detection. The developed system can be stored at room temperature, shows a wide detection range from 1 x 103 to 1 x 107 cells, and enables naked-eye detection with a total detection time of less than 80 min. This allin-one pumpless portable genetic analysis microsystem can be utilized as a simple and accurate platform for POCT diagnostics.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE SA-
dc.titleAll-in-one pumpless portable genetic analysis microsystem for rapid naked-eye detection-
dc.typeArticle-
dc.identifier.wosid000678140800003-
dc.identifier.scopusid2-s2.0-85108104480-
dc.type.rimsART-
dc.citation.volume344-
dc.citation.beginningpage130307-
dc.citation.publicationnameSENSORS AND ACTUATORS B-CHEMICAL-
dc.identifier.doi10.1016/j.snb.2021.130307-
dc.contributor.localauthorIm, Sung Gap-
dc.contributor.nonIdAuthorKim, Yong Tae-
dc.contributor.nonIdAuthorPark, Yoo Min-
dc.contributor.nonIdAuthorLee, Seok Jae-
dc.contributor.nonIdAuthorLee, Kyoung G.-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorPoint-of-care testing-
dc.subject.keywordAuthorMolecular diagnostics-
dc.subject.keywordAuthorPumpless microchip-
dc.subject.keywordAuthorFoodborne pathogens-
dc.subject.keywordAuthorInitiated chemical vapor deposition-
dc.subject.keywordPlusISOTHERMAL AMPLIFICATION-
dc.subject.keywordPlusTESTING SYSTEM-
dc.subject.keywordPlusCHIP-
dc.subject.keywordPlusDEVICE-
dc.subject.keywordPlusPAPER-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusPURIFICATION-
dc.subject.keywordPlusDIAGNOSIS-
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CBE-Journal Papers(저널논문)
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