All-in-One DNA Extraction Tube for Facilitated Real-Time Detection of Infectious Pathogens

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dc.contributor.authorSong, Younseongko
dc.contributor.authorKim, Yong Taeko
dc.contributor.authorChoi, Yunhoko
dc.contributor.authorKim, Hogiko
dc.contributor.authorYeom, Min Heeko
dc.contributor.authorKim, Yesolko
dc.contributor.authorLee, Tae Jaeko
dc.contributor.authorLee, Kyoung G.ko
dc.contributor.authorIm, Sung Gapko
dc.date.accessioned2021-07-30T00:30:55Z-
dc.date.available2021-07-30T00:30:55Z-
dc.date.created2021-06-07-
dc.date.created2021-06-07-
dc.date.created2021-06-07-
dc.date.issued2021-07-
dc.identifier.citationADVANCED HEALTHCARE MATERIALS, v.10, no.14, pp.2100430-
dc.identifier.issn2192-2640-
dc.identifier.urihttp://hdl.handle.net/10203/286931-
dc.description.abstracty An "all-in-one tube" platform is developed, where the genetic analysis involving DNA extraction, amplification, and detection can be performed in a single tube. The all-in-one tube consists of a polymerase chain reaction (PCR) tube in which the inner surface is conformally modified with a tertiary-amine-containing polymer to generate a strong electrostatic interaction with DNA. The all-in-one tube provides high DNA capture efficiency exceeding 80% from Escherichia coli O157: H7 pathogen at a wide range of DNA amount from 0.003 to 3 ng. Indeed, the use of the surface-functionalized PCR tube enables direct amplification and detection of the surface-captured DNA without the modification of standard real-time PCR instrument. Besides, this platform has sensitivity, selectivity, and reliability enough for accurate detection at the minimal infective dose of both gram-positive and negative pathogens. The all-in-one tube enables the direct molecular diagnosis, substantially reducing the labor-intensive pathogen detection steps while providing high compatibility with the currently established real-time PCR instruments, and illustrates its on-site applicability with convenience expandable to various genetic analyses including food safety testing, forensic analysis, and clinical diagnosis.-
dc.languageEnglish-
dc.publisherWILEY-
dc.titleAll-in-One DNA Extraction Tube for Facilitated Real-Time Detection of Infectious Pathogens-
dc.typeArticle-
dc.identifier.wosid000655851700001-
dc.identifier.scopusid2-s2.0-85106686743-
dc.type.rimsART-
dc.citation.volume10-
dc.citation.issue14-
dc.citation.beginningpage2100430-
dc.citation.publicationnameADVANCED HEALTHCARE MATERIALS-
dc.identifier.doi10.1002/adhm.202100430-
dc.contributor.localauthorIm, Sung Gap-
dc.contributor.nonIdAuthorKim, Yong Tae-
dc.contributor.nonIdAuthorYeom, Min Hee-
dc.contributor.nonIdAuthorKim, Yesol-
dc.contributor.nonIdAuthorLee, Tae Jae-
dc.contributor.nonIdAuthorLee, Kyoung G.-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorall-in-one tubes-
dc.subject.keywordAuthornucleic acid extraction-
dc.subject.keywordAuthorpathogen detection-
dc.subject.keywordAuthorreal-time polymerase chain reaction-
dc.subject.keywordAuthortertiary-amine-containing functional polymers-
dc.subject.keywordPlusNUCLEIC-ACID EXTRACTION-
dc.subject.keywordPlusAMPLIFICATION-
dc.subject.keywordPlusPLATFORM-
dc.subject.keywordPlusLAB-
dc.subject.keywordPlusCAPILLARY-
dc.subject.keywordPlusPCR-
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