DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Younseong | ko |
dc.contributor.author | Kim, Yong Tae | ko |
dc.contributor.author | Choi, Yunho | ko |
dc.contributor.author | Kim, Hogi | ko |
dc.contributor.author | Yeom, Min Hee | ko |
dc.contributor.author | Kim, Yesol | ko |
dc.contributor.author | Lee, Tae Jae | ko |
dc.contributor.author | Lee, Kyoung G. | ko |
dc.contributor.author | Im, Sung Gap | ko |
dc.date.accessioned | 2021-07-30T00:30:55Z | - |
dc.date.available | 2021-07-30T00:30:55Z | - |
dc.date.created | 2021-06-07 | - |
dc.date.created | 2021-06-07 | - |
dc.date.created | 2021-06-07 | - |
dc.date.issued | 2021-07 | - |
dc.identifier.citation | ADVANCED HEALTHCARE MATERIALS, v.10, no.14, pp.2100430 | - |
dc.identifier.issn | 2192-2640 | - |
dc.identifier.uri | http://hdl.handle.net/10203/286931 | - |
dc.description.abstract | y An "all-in-one tube" platform is developed, where the genetic analysis involving DNA extraction, amplification, and detection can be performed in a single tube. The all-in-one tube consists of a polymerase chain reaction (PCR) tube in which the inner surface is conformally modified with a tertiary-amine-containing polymer to generate a strong electrostatic interaction with DNA. The all-in-one tube provides high DNA capture efficiency exceeding 80% from Escherichia coli O157: H7 pathogen at a wide range of DNA amount from 0.003 to 3 ng. Indeed, the use of the surface-functionalized PCR tube enables direct amplification and detection of the surface-captured DNA without the modification of standard real-time PCR instrument. Besides, this platform has sensitivity, selectivity, and reliability enough for accurate detection at the minimal infective dose of both gram-positive and negative pathogens. The all-in-one tube enables the direct molecular diagnosis, substantially reducing the labor-intensive pathogen detection steps while providing high compatibility with the currently established real-time PCR instruments, and illustrates its on-site applicability with convenience expandable to various genetic analyses including food safety testing, forensic analysis, and clinical diagnosis. | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.title | All-in-One DNA Extraction Tube for Facilitated Real-Time Detection of Infectious Pathogens | - |
dc.type | Article | - |
dc.identifier.wosid | 000655851700001 | - |
dc.identifier.scopusid | 2-s2.0-85106686743 | - |
dc.type.rims | ART | - |
dc.citation.volume | 10 | - |
dc.citation.issue | 14 | - |
dc.citation.beginningpage | 2100430 | - |
dc.citation.publicationname | ADVANCED HEALTHCARE MATERIALS | - |
dc.identifier.doi | 10.1002/adhm.202100430 | - |
dc.contributor.localauthor | Im, Sung Gap | - |
dc.contributor.nonIdAuthor | Kim, Yong Tae | - |
dc.contributor.nonIdAuthor | Yeom, Min Hee | - |
dc.contributor.nonIdAuthor | Kim, Yesol | - |
dc.contributor.nonIdAuthor | Lee, Tae Jae | - |
dc.contributor.nonIdAuthor | Lee, Kyoung G. | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | all-in-one tubes | - |
dc.subject.keywordAuthor | nucleic acid extraction | - |
dc.subject.keywordAuthor | pathogen detection | - |
dc.subject.keywordAuthor | real-time polymerase chain reaction | - |
dc.subject.keywordAuthor | tertiary-amine-containing functional polymers | - |
dc.subject.keywordPlus | NUCLEIC-ACID EXTRACTION | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | LAB | - |
dc.subject.keywordPlus | CAPILLARY | - |
dc.subject.keywordPlus | PCR | - |
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