Ultrafast and Real-Time Nanoplasmonic On- Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics

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Advent and fast spread of pandemic diseases draw worldwide attention to rapid, prompt, and accurate molecular diagnostics with technical development of ultrafast polymerase chain reaction (PCR). Microfluidic on-chip PCR platforms provide highly efficient and small-volume bioassay for point-of-care diagnostic applications. Here we report ultrafast, real-time, and on-chip nanoplasmonic PCR for rapid and quantitative molecular diagnostics at point-of-care level. The plasmofluidic PCR chip comprises glass nanopillar arrays with Au nanoislands and gas-permeable microfluidic channels, which contain reaction microchamber arrays, a precharged vacuum cell, and a vapor barrier. The on-chip configuration allows both spontaneous sample loading and microbubble-free PCR reaction during which the plasmonic nanopillar arrays result in ultrafast photothermal cycling. After rapid sample loading less than 3 min, two-step PCR results for 40 cycles show rapid amplification in 264 s for lambda-DNA, and 306 s for plasmids expressing SARS-CoV-2 envelope protein. In addition, the in situ cyclic real-time quantification of amplicons clearly demonstrates the amplification efficiencies of more than 91%. This PCR platform can provide rapid point-of-care molecular diagnostics in helping slow the fast-spreading pandemic.
Publisher
AMER CHEMICAL SOC
Issue Date
2021-06
Language
English
Article Type
Article
Citation

ACS NANO, v.15, no.6, pp.10194 - 10202

ISSN
1936-0851
DOI
10.1021/acsnano.1c02154
URI
http://hdl.handle.net/10203/286727
Appears in Collection
BiS-Journal Papers(저널논문)
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