DC Field | Value | Language |
---|---|---|
dc.contributor.author | Koo, Bon Il | ko |
dc.contributor.author | Choi, Jae Woong | ko |
dc.contributor.author | Song, Seuk Young | ko |
dc.contributor.author | Choi, Ye Hun | ko |
dc.contributor.author | Lee, Tae Yong | ko |
dc.contributor.author | Kim, Shin-Hyun | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.contributor.author | Nam, Yoon Sung | ko |
dc.date.accessioned | 2021-06-23T02:30:19Z | - |
dc.date.available | 2021-06-23T02:30:19Z | - |
dc.date.created | 2021-04-26 | - |
dc.date.issued | 2021-06 | - |
dc.identifier.citation | ADVANCED FUNCTIONAL MATERIALS, v.31, no.25, pp.2102497 | - |
dc.identifier.issn | 1616-301X | - |
dc.identifier.uri | http://hdl.handle.net/10203/286110 | - |
dc.description.abstract | Mineral carbonation is the most effective carbon capture technique, but carbon dioxide (CO2) conversion is limited by the slow hydration rate of CO2 (<10(-1) s(-1)). A biological solution exists: carbonic anhydrase (CA) efficiently hydrates CO2 at a turnover rate of approximate to 10(6) s(-1) under ambient conditions, making it an extremely attractive candidate for industrial post-combustion CO2 capture. However, high cost and poor long-term stability impose a technical barrier to its practical uses. Here, a genetically engineered Corynebacterium glutamicum (C. glutamicum) is introduced as a robust cell display platform for the in situ stabilization and low-cost production of CA. The enzyme is displayed in the mycolic layer with porin B as an anchoring protein with (GGGGS)(2) as a spacer. The cell-displayed CA exhibits no significant inactivation of the CO2 hydration activity for at least one month at 37 degrees C. Its denaturation rate constant at 50 degrees C (0.07) is an order of magnitude lower than that of free CA (0.52-0.54). This study demonstrates that a structurally robust cell template allows the effective stabilization of CA, suggesting the C. glutamicum-based cell display as a promising technique to achieve highly efficient, sustainable, and low-cost CO2 capture for industrial applications. | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Robust Biocatalysts Displayed on Crystalline Protein-Layered Cells for Efficient and Sustainable Hydration of Carbon Dioxide | - |
dc.type | Article | - |
dc.identifier.wosid | 000640566600001 | - |
dc.identifier.scopusid | 2-s2.0-85104312009 | - |
dc.type.rims | ART | - |
dc.citation.volume | 31 | - |
dc.citation.issue | 25 | - |
dc.citation.beginningpage | 2102497 | - |
dc.citation.publicationname | ADVANCED FUNCTIONAL MATERIALS | - |
dc.identifier.doi | 10.1002/adfm.202102497 | - |
dc.contributor.localauthor | Kim, Shin-Hyun | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.localauthor | Nam, Yoon Sung | - |
dc.contributor.nonIdAuthor | Song, Seuk Young | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | carbon dioxide capture | - |
dc.subject.keywordAuthor | carbonic anhydrase | - |
dc.subject.keywordAuthor | cell display | - |
dc.subject.keywordAuthor | Corynebacterium | - |
dc.subject.keywordAuthor | enzyme thermostability | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.