Expression and purification of toll-like receptor 3 = 톨 유사 수용체 3 발현 및 정제

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Toll-like receptors(TLRs) play key roles in the initiation of the innate immune system by recognition of conserved foreign molecules called pathogen-associated molecular patterns. TLR 3 is located in endosomes and it recognizes virus infection by sensing dsRNA which is produced during virus replication. Especially, TLR3 is related to CNS virus infection. However, the activation mechanism and downstream signaling pathways are unclear because of considering only ectodomain and lack of complete structural information. In this research, we focused on expression and purification of full-length of TLR3 which is essential for revealing activation mechanisms by structural studies. To provided correct post-translation modification and lipid environments, TLR3 was expressed by the mammalian expression system – bacmam. Solubilization detergent that is one of the most important for membrane protein stability could be determined by FSEC. Finally, the sample quality was check by negative-stained TEM.
Advisors
Kang, Jin Youngresearcher강진영researcher
Description
한국과학기술원 :화학과,
Publisher
한국과학기술원
Issue Date
2020
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 화학과, 2020.2,[iii, 34 p. :]

Keywords

Toll-like receptor 3▼amembrane receptor▼aexpression▼apurification▼anegative stain electron microscopy; 톨 유사 수용체 3▼a세포막수용체▼a발현▼a정제▼a음성 염색 전자현미경

URI
http://hdl.handle.net/10203/283774
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=901476&flag=dissertation
Appears in Collection
CH-Theses_Master(석사논문)
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