Formation of $\TiO_2$ nanocomposites and thin films by a rationally designed $(RKK)_4D_8$ peptide$(RKK)_4D_8$ 펩타이드를 이용한 산화티타늄 복합체 및 박막 형성 연구

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Biomineralization enables control over organic/inorganic composites and thin film formation on the nanometric scale. The minerals form under ambient conditions, and can therefore be applied for biological research. In this research, organic/inorganic composites/thin film formation, its mechanism, and its applications to cell surfaces were investigated. Arginine (R) and lysine (K) peptides are known to efficiently form $\TiO_2$ under ambient conditions. Using $(RKK)_4(D)_8$ peptides as a template (aspartic acid (D) was added to improve cytocompatibility), the mechanism of $\TiO_2$ formation was explored, and the determining factor of crystallinity was analyzed. Ultimately, the $(RKK)_4(D)_8$ peptide was found not to be a catalyst, but inducer in the reaction. Afterwards, biomineralization on living cell surfaces using the $(RKK)_4(D)_8$ peptide was conducted. Biomineralization of the naturally present cell membrane results in the creation of robust artificial cell structures with novel structures and properties. For cell encapsulation, all materials and methods should be cytocompatible, and the artificially-made shell should be both durable and permeable for maintaining viability. These newly formed entities are the result of interfacial reactions between the fragile cellular membrane and tough artificial shells. Through this work, we can protect cells from harmful external stresses, much like artificial spores found in nature, as well as ensure their long-term preservation. First, living Chlorella cells were encapsulated individually with abiological, functionalizable $\TiO_2$. The bioinspired cytocompatible reaction conditions allowed the encapsulated Chlorella cells to maintain their viability and original shapes. After formation of the $\TiO_2$ shells, the shells were post-functionalized by using catechol chemistry. Second, individual Chlorella cells were encapsulated within a $SiO_2$-$\TiO_2$ nanoshell with high cell viability. The encapsulated Chlorella showed thermo-tolerance due to the heat dissipative property of the inorganic material. Mammalian cell encapsulation is difficult because of their innate fragility, however, encapsulation of mammalian Jurkat T cells in artificial $\TiO_2$ shells was achieved. The artificially-made $\TiO_2$ shells were maintained for more than 3 days, and granted long-term thermoprotection. Also, encapsulated cells maintained their immunoreactive properties after the encapsulation processes.
Advisors
Choi, Insungresearcher최인성researcher
Description
한국과학기술원 :화학과,
Publisher
한국과학기술원
Issue Date
2016
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 화학과, 2016.8,[ix, 75 p. :]

Keywords

Organic/Inorganic Nanocomposites▼aOrganic/Inorganic Thin Film▼aBiomineralization▼aCell Encapsulation▼aHeat Dissipation; 유/무기 복합체▼a유/무기 박막▼a생광물화반응▼a세포피포화▼a방열 효과

URI
http://hdl.handle.net/10203/283422
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=886598&flag=dissertation
Appears in Collection
CH-Theses_Ph.D.(박사논문)
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