Microarray-integrated optoelectrofluidic system for biological assay생물학적 분석을 위한 마이크로어레이가 집적된 광전자유체 시스템 개발

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dc.contributor.advisorPark, Je-Kyun-
dc.contributor.advisor박제균-
dc.contributor.authorHan, Dongsik-
dc.date.accessioned2021-05-11T19:40:56Z-
dc.date.available2021-05-11T19:40:56Z-
dc.date.issued2016-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=886599&flag=dissertationen_US
dc.identifier.urihttp://hdl.handle.net/10203/283421-
dc.description학위논문(박사) - 한국과학기술원 : 바이오및뇌공학과, 2016.8,[xi, 123 p. :]-
dc.description.abstractThis thesis presents the development of an integrated optoelectrofluidic system for biological assays based on a microarray format to overcome low reaction efficiency, a significant issue of surface-based sensing system. An analyte depletion problem commonly occurs in immunoassay systems where mass transport of target molecules depends on only diffusion manner, which causes a loss of assay performances. To improve the reaction efficiency, active mass transport of molecules at assay regions was generated by applying an optically-induced AC electrokinetic flow, resulting in an achievement of enhanced immunoassay results. To understand the optoelectrofluidic mass transport, I firstly investigated the effect of an optically-induced AC electroosmosis (ACEO) on focusing colloidal particles. Colloidal particles could be concentrated to a projected region thanks to the optically-induced strong convective flow. Based on these studies, I explored potential of the optoelectrofluidic technology to be effectively utilized in a microfluidic environment where mass transport is limited. Second, I proposed an optoelectrofluidic enhanced immunoreaction based on the optically-induced ACEO. Mass transport phenomena of molecules under the optoelectrofluidic system with a surface-based assay system were investigated by mathematical simulation studies and experiments using an immunoreaction set. The proposed system with an optically-induced convective flow could improve the reaction efficiency with a 2.18-fold enhancement under dynamic light patterns compared to the passive mode by overcoming the diffusion limitation problem. Finally, I presented a novel optoelectrofluidic immunoassay integrated with a microarray format. Multiple convective flows were generated on multiple spots of the microarray by an optical manner, which enhanced mass transport, resulting in a reduction of the reaction time from more than 30 min to 10 min with minimal sample consumption. Based on this enhancing effect, the microarray-integrated optoelectrofluidic system was applied in quantitative immunoassay. Furthermore, the application of multiplex immunoassay was demonstrated by simultaneous multiple protein detection.-
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectImmunoassay▼aMicroarray▼aMicrofluidic focusing▼aMicrofluidics▼aOptoelectrofluidics-
dc.subject면역분석법▼a마이크로어레이▼a미세유체역학 집속▼a미세유체역학▼a광전자유체역학-
dc.titleMicroarray-integrated optoelectrofluidic system for biological assay-
dc.title.alternative생물학적 분석을 위한 마이크로어레이가 집적된 광전자유체 시스템 개발-
dc.typeThesis(Ph.D)-
dc.identifier.CNRN325007-
dc.description.department한국과학기술원 :바이오및뇌공학과,-
dc.contributor.alternativeauthor한동식-
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