DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Kang, Jin Young | - |
dc.contributor.advisor | 강진영 | - |
dc.contributor.author | Lee, Ji Min | - |
dc.date.accessioned | 2021-05-11T19:34:58Z | - |
dc.date.available | 2021-05-11T19:34:58Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=875558&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/283132 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 화학과, 2019.8,[iv, 38 p. :] | - |
dc.description.abstract | Analyzing the 3D structure of a protein is fundamental in understanding the function of the protein and its interaction with other biomolecules. Recently, protein structure analysis using the cryo-EM has become prevalent among structural biologists due to the rapid advances in microscopes, detectors and the computer software that process the data. However, elucidating the 3D structure of a small-sized protein is still a challenge. There have been many different approaches to solve this problem. One of the solutions is to develop a scaffolding system that is relatively bigger in size than the target protein. By linking the small-sized target protein to the relatively bigger scaffolding system, imaging is expected to become considerably easier. In this research, we designed and tested a novel scaffolding system based on antibodies. Antibodies are connected to other proteins of renowned protein interactions to create a new protein complex. By changing the types of antibodies of the scaffolding system, we hope to develop a universal scaffolding system appropriate for analyzing 3D structures of various small-sized proteins. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | electron microscope▼aantibody▼aprotein interaction▼ascaffolding system▼asmall-sized target proteins | - |
dc.subject | 전자현미경▼a항체▼a단백질 상호작용▼a스캐폴딩 시스템▼a작은 크기 표적 단백질 | - |
dc.title | (A) design of antibody-based scaffolding system for structure analysis of small-sized proteins in EM | - |
dc.title.alternative | 전자현미경을 이용한 작은 크기의 단백질 구조 분석을 위한 항체기반 스캐폴딩 시스템 설계 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :화학과, | - |
dc.contributor.alternativeauthor | 이지민 | - |
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