CRISPR nanocomplex for nonviral genome editing and method for preparing the same

Abstract

Abstract The present invention relates to a CRISPR nanocomplex for nonviral genome editing, a method for preparing the same, and the like. The CRISPR nanocomplex 5 for nonviral genome editing of the present invention has a size of several nanometers to several microns, enables intracellular delivery without external physical stimulation, and can be utilized for genome editing through nonviral routes with respect to target genes of 10 cells. As a result, when used for preparation of animal model, microbiological engineering, cell engineering for disease treatment, or formulations for biological administration, the CRISPR Nanocomplex shows high intracellular delivery and gene editing efficiency, and 15 can minimize problems, such as nonspecific editing, gene mutation, and induction of cytotoxicity and biotoxicity.