Novel culture system via wirelessly controllable optical stimulation of the FGF signaling pathway for human and pig pluripotency

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Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation of pluripotent stem cells (PSCs) and result in significant costs in large-scale manufacturing. Here, we report a novel PSC culture system via wirelessly controllable optical activation of the fibroblast growth factor (FGF) signaling pathway without the need for supplementation of recombinant FGF2 protein, a key molecule for maintaining pluripotency of PSCs. Using a fusion protein between the cytoplasmic region of the FGF receptor-1 and a light-oxygen-voltage domain, we achieved tunable, blue light-dependent activation of FGF signaling in human and porcine PSCs. Our data demonstrate that a highly controllable optical stimulation of the FGF signaling pathway is sufficient for long-term maintenance of PSCs, without the loss of differentiation potential into three germ layers. This culture system will be a cost-effective platform for a large-scale stem cell culture.
Publisher
ELSEVIER SCI LTD
Issue Date
2021-02
Language
English
Article Type
Article
Citation

BIOMATERIALS, v.269

ISSN
0142-9612
DOI
10.1016/j.biomaterials.2020.120222
URI
http://hdl.handle.net/10203/281593
Appears in Collection
BS-Journal Papers(저널논문)
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