Crystal structure of PYCH_01220 from Pyrococcus yayanosii potentially involved in binding nucleic acid

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We report the crystal structure of PYCH_01220, a hypothetical protein in Pyrococcus yayanosii CH1. This protein is composed of two domains, named Domain A and Domain B. While Domain B is not significantly homologous to known protein structures, Domain A is structurally analogous to the C-terminal ribonuclease domain of Escherichia coli colicin D. Domain A has a positively charged surface patch rendered by 13 basic residues, eight arginine or lysine residues of which are evolutionarily conserved. Electrophoretic mobility shift assays showed that PYCH_01220 binds to DNA, and charge-inversion mutations on this patch negatively affect the DNA binding, suggesting that the function of PYCH_01220 might involve nucleic acid-binding via the positively charged patch.
Publisher
Wiley-Liss Inc
Issue Date
2021-04
Language
English
Article Type
Article
Citation

Proteins: Structure, Function and Genetics, v.89, no.4, pp.468 - 472

ISSN
0887-3585
DOI
10.1002/prot.26029
URI
http://hdl.handle.net/10203/281178
Appears in Collection
BS-Journal Papers(저널논문)
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