DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, S. Chul | ko |
dc.contributor.author | Baek, S. Chan | ko |
dc.contributor.author | Choi, Yeon-Gil | ko |
dc.contributor.author | Yang, Jihye | ko |
dc.contributor.author | Lee, Young-suk | ko |
dc.contributor.author | Woo, Jae-Sung | ko |
dc.contributor.author | Kim, V. Narry | ko |
dc.date.accessioned | 2021-02-01T01:10:15Z | - |
dc.date.available | 2021-02-01T01:10:15Z | - |
dc.date.created | 2021-02-01 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.citation | MOLECULAR CELL, v.73, no.3, pp.505 - + | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | http://hdl.handle.net/10203/280415 | - |
dc.description.abstract | Microprocessor, composed of DROSHA and its cofactor DGCR8, initiates microRNA(miRNA) biogenesis by processing the primary transcripts of miRNA (pri-miRNAs). Here we investigate the mechanism by which Microprocessor selects the cleavage site with single-nucleotide precision, which is crucial for the specificity and functionality of miRNAs. By testing similar to 40,000 pri-miRNA variants, we find that for some pri-miRNAs the cleavage site is dictated mainly by the mGHG motif embedded in the lower stem region of pri-miRNA. Structural modeling and deep-sequencing-based complementation experiments show that the double-stranded RNA-binding domain (dsRBD) of DROSHA recognizes mGHG to place the catalytic center in the appropriate position. The mGHG motif as well as the mGHG-recognizing residues in DROSHA dsRBD are conserved across eumetazoans, suggesting that this mechanism emerged in an early ancestor of the animal lineage. Our findings provide a basis for the understanding of miRNA biogenesis and rational design of accurate small-RNA-based gene silencing. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | Molecular Basis for the Single-Nucleotide Precision of Primary microRNA Processing | - |
dc.type | Article | - |
dc.identifier.wosid | 000458015200011 | - |
dc.identifier.scopusid | 2-s2.0-85061010954 | - |
dc.type.rims | ART | - |
dc.citation.volume | 73 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 505 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | MOLECULAR CELL | - |
dc.identifier.doi | 10.1016/j.molcel.2018.11.005 | - |
dc.contributor.localauthor | Lee, Young-suk | - |
dc.contributor.nonIdAuthor | Kwon, S. Chul | - |
dc.contributor.nonIdAuthor | Baek, S. Chan | - |
dc.contributor.nonIdAuthor | Choi, Yeon-Gil | - |
dc.contributor.nonIdAuthor | Yang, Jihye | - |
dc.contributor.nonIdAuthor | Woo, Jae-Sung | - |
dc.contributor.nonIdAuthor | Kim, V. Narry | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RNA-INTERFERENCE | - |
dc.subject.keywordPlus | RIBONUCLEASE-III | - |
dc.subject.keywordPlus | SEQUENCE DETERMINANTS | - |
dc.subject.keywordPlus | READ ALIGNMENT | - |
dc.subject.keywordPlus | DICER | - |
dc.subject.keywordPlus | DROSHA | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | PRECURSORS | - |
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