Solvent-Free Photobiocatalytic Hydroxylation of Cyclohexane

Cited 3 time in webofscience Cited 0 time in scopus
  • Hit : 136
  • Download : 31
The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase fromAgrocybe aegerita(AaeUPO) can hydroxylate the neat model substrate cyclohexane. H(2)O(2)was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N-CNDs) and UV LED illumination.AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N-CNDs absorption in beads containingAaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2020-08
Language
English
Article Type
Article
Citation

CHEMCATCHEM, v.12, no.16, pp.4009 - 4013

ISSN
1867-3880
DOI
10.1002/cctc.202000512
URI
http://hdl.handle.net/10203/277114
Appears in Collection
MS-Journal Papers(저널논문)
Files in This Item
000539635000001.pdf(2.07 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 3 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0