Formation and functionalization of membraneless compartments inEscherichia coli

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dc.contributor.authorWei, Shao-Pengko
dc.contributor.authorQian, Zhi-Gangko
dc.contributor.authorHu, Chun-Feiko
dc.contributor.authorPan, Fangko
dc.contributor.authorChen, Meng-Tingko
dc.contributor.authorLee, Sang Yupko
dc.contributor.authorXia, Xiao-Xiako
dc.date.accessioned2020-09-21T06:55:03Z-
dc.date.available2020-09-21T06:55:03Z-
dc.date.created2020-07-15-
dc.date.issued2020-10-
dc.identifier.citationNATURE CHEMICAL BIOLOGY, v.16, no.10, pp.1143 - 1148-
dc.identifier.issn1552-4450-
dc.identifier.urihttp://hdl.handle.net/10203/276367-
dc.description.abstractMembraneless organelles formed by liquid-liquid phase separation of proteins or nucleic acids are involved in diverse biological processes in eukaryotes. However, such cellular compartments have yet to be discovered or created synthetically in prokaryotes. Here, we report the formation of liquid protein condensates inside the cells of prokaryoticEscherichia coliupon heterologous overexpression of intrinsically disordered proteins such as spider silk and resilin. In vitro reconstitution under conditions that mimic intracellular physiologically crowding environments ofE. colirevealed that the condensates are formed via liquid-liquid phase separation. We also show functionalization of these condensates via targeted colocalization of cargo proteins to create functional membraneless compartments able to fluoresce and to catalyze biochemical reactions. The ability to form and functionalize membraneless compartments may serve as a versatile tool to develop artificial organelles with on-demand functions in prokaryotes for applications in synthetic biology. Utilization of structural proteins with intrinsically disordered regions enables the formation of membraneless organelles inEscherichia colithrough liquid-liquid phase separation, as well as their functionalization with active enzymes.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.titleFormation and functionalization of membraneless compartments inEscherichia coli-
dc.typeArticle-
dc.identifier.wosid000544153200001-
dc.identifier.scopusid2-s2.0-85087039969-
dc.type.rimsART-
dc.citation.volume16-
dc.citation.issue10-
dc.citation.beginningpage1143-
dc.citation.endingpage1148-
dc.citation.publicationnameNATURE CHEMICAL BIOLOGY-
dc.identifier.doi10.1038/s41589-020-0579-9-
dc.contributor.localauthorLee, Sang Yup-
dc.contributor.nonIdAuthorWei, Shao-Peng-
dc.contributor.nonIdAuthorQian, Zhi-Gang-
dc.contributor.nonIdAuthorHu, Chun-Fei-
dc.contributor.nonIdAuthorPan, Fang-
dc.contributor.nonIdAuthorChen, Meng-Ting-
dc.contributor.nonIdAuthorXia, Xiao-Xia-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle; Early Access-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusPHASE-SEPARATION-
dc.subject.keywordPlusDISORDERED PROTEINS-
dc.subject.keywordPlusORGANELLES-
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