DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dasom | ko |
dc.contributor.author | Seo, Hyo-Deok | ko |
dc.contributor.author | Ryu, Yiseul | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2020-09-18T03:58:51Z | - |
dc.date.available | 2020-09-18T03:58:51Z | - |
dc.date.created | 2020-09-07 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.citation | ANALYTICA CHIMICA ACTA, v.1126, pp.154 - 162 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | http://hdl.handle.net/10203/276075 | - |
dc.description.abstract | The quest for highly sensitive and specific detection of disease biomarkers is high, despite many advances in analysis system. Here, we present a sensitive immunoassay platform using DNA-tethered gold nanoparticles and DNA-binding zinc fingers (ZFs). Monomeric alkaline phosphatase (mAP) and human TNF-alpha were employed as a signal generator and a disease biomarker, respectively. Gold nanoparticles (AuNPs) were first grafted with double-stranded DNAs having specific sequences for two different types of ZFs (QNK and zif268). The alkaline phosphatase and TNF-alpha-specific protein binder were genetically fused to each of two different types of ZFs, respectively, followed by conjugation with the DNA-tethered AuNPs in a sequence-specific manner. The use of the functionalized AuNPs as a signal generator in a colorimetric immunoassay of TNF-alpha led to LOD of 120 pg/ml, showing about 161-fold higher sensitivity than a protein binder-fused mAP. The present immunoassay platform could be applied to other analytes by simply replacing a targeting moiety, allowing a versatile and reproducible colorimetric immunoassay. | - |
dc.language | English | - |
dc.publisher | ELSEVIER | - |
dc.title | Functionalized gold nanoparticles with zinc finger-fused proteins as a colorimetric immunoassay platform | - |
dc.type | Article | - |
dc.identifier.wosid | 000561629700018 | - |
dc.identifier.scopusid | 2-s2.0-85087916418 | - |
dc.type.rims | ART | - |
dc.citation.volume | 1126 | - |
dc.citation.beginningpage | 154 | - |
dc.citation.endingpage | 162 | - |
dc.citation.publicationname | ANALYTICA CHIMICA ACTA | - |
dc.identifier.doi | 10.1016/j.aca.2020.06.019 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Colorimetric immunoassay | - |
dc.subject.keywordAuthor | Monomeric alkaline phosphatase (mAP) | - |
dc.subject.keywordAuthor | Gold nanoparticles (AuNPs) | - |
dc.subject.keywordAuthor | Zinc fingers (ZFs) | - |
dc.subject.keywordAuthor | Repebody | - |
dc.subject.keywordPlus | CANCER BIOMARKER DETECTION | - |
dc.subject.keywordPlus | CONFORMATIONAL-CHANGES | - |
dc.subject.keywordPlus | BINDING-AFFINITY | - |
dc.subject.keywordPlus | AVIDITY | - |
dc.subject.keywordPlus | ELISA | - |
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