Functionalized gold nanoparticles with zinc finger-fused proteins as a colorimetric immunoassay platform

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dc.contributor.authorKim, Dasomko
dc.contributor.authorSeo, Hyo-Deokko
dc.contributor.authorRyu, Yiseulko
dc.contributor.authorKim, Hak-Sungko
dc.date.accessioned2020-09-18T03:58:51Z-
dc.date.available2020-09-18T03:58:51Z-
dc.date.created2020-09-07-
dc.date.issued2020-08-
dc.identifier.citationANALYTICA CHIMICA ACTA, v.1126, pp.154 - 162-
dc.identifier.issn0003-2670-
dc.identifier.urihttp://hdl.handle.net/10203/276075-
dc.description.abstractThe quest for highly sensitive and specific detection of disease biomarkers is high, despite many advances in analysis system. Here, we present a sensitive immunoassay platform using DNA-tethered gold nanoparticles and DNA-binding zinc fingers (ZFs). Monomeric alkaline phosphatase (mAP) and human TNF-alpha were employed as a signal generator and a disease biomarker, respectively. Gold nanoparticles (AuNPs) were first grafted with double-stranded DNAs having specific sequences for two different types of ZFs (QNK and zif268). The alkaline phosphatase and TNF-alpha-specific protein binder were genetically fused to each of two different types of ZFs, respectively, followed by conjugation with the DNA-tethered AuNPs in a sequence-specific manner. The use of the functionalized AuNPs as a signal generator in a colorimetric immunoassay of TNF-alpha led to LOD of 120 pg/ml, showing about 161-fold higher sensitivity than a protein binder-fused mAP. The present immunoassay platform could be applied to other analytes by simply replacing a targeting moiety, allowing a versatile and reproducible colorimetric immunoassay.-
dc.languageEnglish-
dc.publisherELSEVIER-
dc.titleFunctionalized gold nanoparticles with zinc finger-fused proteins as a colorimetric immunoassay platform-
dc.typeArticle-
dc.identifier.wosid000561629700018-
dc.identifier.scopusid2-s2.0-85087916418-
dc.type.rimsART-
dc.citation.volume1126-
dc.citation.beginningpage154-
dc.citation.endingpage162-
dc.citation.publicationnameANALYTICA CHIMICA ACTA-
dc.identifier.doi10.1016/j.aca.2020.06.019-
dc.contributor.localauthorKim, Hak-Sung-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorColorimetric immunoassay-
dc.subject.keywordAuthorMonomeric alkaline phosphatase (mAP)-
dc.subject.keywordAuthorGold nanoparticles (AuNPs)-
dc.subject.keywordAuthorZinc fingers (ZFs)-
dc.subject.keywordAuthorRepebody-
dc.subject.keywordPlusCANCER BIOMARKER DETECTION-
dc.subject.keywordPlusCONFORMATIONAL-CHANGES-
dc.subject.keywordPlusBINDING-AFFINITY-
dc.subject.keywordPlusAVIDITY-
dc.subject.keywordPlusELISA-
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