Functional consequences of $IP_3$ receptor polymorphism = $IP_3$ 수용체의 다양성과 기능 변화

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Anxiety in mice can be measured by behavioral reactivity to social or non-social stressors. These behaviors were compared by performing the resident-intruder test (social) as well as the light-dark transition and open-field tests (non-social) for the FVB, C57BL/6, and BALB/c lines of mouse. The three inbred lines showed significant differences in their responses to intruder mice. In order to obtain common factors, a principal component analysis was performed. Three factors, accounting for about 68 % of the total variance, were extracted from the scores obtained from the three behavioral tests. The first two major factors are primarily associated with the anxiety-related behaviors. One includes anxiety behaviors with a locomotive basis, while the other includes defecation measured in both anxiety tests. The third factor explains the three social behaviors, facial investigation, ano-genital investigation, and following, observed in the resident intruder test, although facial investigation is also moderately associated with the second factor. The results indicate that the behavioral responses to an intruder share a component distinct from anxiety-related behaviors. BALB/c mice showed a higher defecation than C57BL/6 under stress condition. Inositol 1,4,5-trisphosphate receptor $(IP_3R)$ regulates defecation rhythm in C.elegans. Thus, in order to investigate that the phenotypic variation is associated with functional differences of $Type 3 IP_3R (IP_3R3)$, which is major $IP_3R$ isofrom in the intestine, polymorphism was searched. Here, the natural $Pro^{335}Leu$ polymorphism of mouse $IP_3R_3$ was found between BABL/c and C57BL/6J. The functional differences between $Pro^{335}IP_3R_3$ and $Leu^{335}IP_3R_3$ were investigated with purified receptors reconstituted into proteoliposomes as well as with soluble ligand binding domains (LBDs). $Pro^{335}IP_3R_3$ exhibited significantly higher $IP_3-binding$ affinity and $IP_3-induced Ca^{2+}$ release than those of $Leu^...
Advisors
Park, Chan-Kyuresearcher박찬규researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
249369/325007  / 000985065
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2005.8, [ viii, 101 p. ]

Keywords

anxiety; inbred mouse; ligand-binding affinity; 리간드 부착 친화도; 정서성; 생쥐모델

URI
http://hdl.handle.net/10203/27595
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=249369&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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