Identification of an enhancer region in the mouse ephA8 locus directing expression to the anterior region of dorsal mesencephalon = 생쥐 중뇌의 전배위에서 이피에이치 에이8(ephA8) 유전자 발현을 조절하는 인핸서(enhancer) 규명

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Eph receptors and ephrins are dynamically expressed in a wide range of regions of the vertebrate during embryogenesis. The dorsal mesencephalon is segmented into two broad regions demarcated by the mutually exclusive expression of EphA receptors and ephrinA ligands. It is of considerable interest to elucidate how these expression domains are established in the development of the mesencephalon. In this study, we employed a transgenic approach to define the cis-acting DNA regulatory elements involved in the anterior mesencephalon-specific expression of the mouse ephAB gene. Our analyses of the temporal and spatial expression patterns of various ephA8/lacZ gene fusions in transgenic mice revealed that the 10 kb genomic DNA 5`` immediately upstream of the ephAB coding sequence is capable of directing lacZ expression in an ephA8-specific manner. Further deletion analyses of the ephA8 genomic region led to the identification of a 1 kb enhancer region, which directs expression in the embryo to the anterior region of the developing midbrain. This ephA8-specific regulatory DNA sequences can now be used in biochemical analyses to identify proteins modulating the anterior differentiation of the optic tectum, and in functional analyses to direct the expression of other developmentally important genes to this region
Yoo, Ook-Joonresearcher유욱준researcher
한국과학기술원 : 생물과학과,
Issue Date
177213/325007 / 000985018

학위논문(박사) - 한국과학기술원 : 생물과학과, 2002.8, [ viii, 104 p. ]


ephA8 enhancer mesencephalon dorsal anterior; gene expression mouse tectum; 유전자 발현 생쥐 텍튬; 중뇌 전배위 이피에이치에이8 인핸서

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