DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sohn, Yoo-Kyoung | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2020-05-20T08:20:05Z | - |
dc.date.available | 2020-05-20T08:20:05Z | - |
dc.date.created | 2020-05-18 | - |
dc.date.created | 2020-05-18 | - |
dc.date.created | 2020-05-18 | - |
dc.date.created | 2020-05-18 | - |
dc.date.issued | 2020-05 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.526, no.2, pp.447 - 452 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/274244 | - |
dc.description.abstract | Bcl-2 family proteins are critical switches to control cell death and survival, and Bcl-2 is a key regulator in pro-survival signaling, causing various diseases including cancers. Bcl-2 has drawn a considerable attention as a potential target for developing a pro-apoptotic agent for cancers. We here present the development of a specific protein binder against human Bcl-2 and its cytosolic delivery to effectively induce apoptosis of cancer cells. The protein binder composed of leucine-rich repeat modules was selected for human Bcl-2, and its binding affinity was increased up to 60 nM through a modular evolution-based approach. The protein binder was efficiently delivered into cancer cells by an intracellular delivery system using a translocation domain from a bacterial exotoxin, resulting in a strong suppression of anti-apoptotic signaling in cancer cells. Our results demonstrate that the human Bcl-2-specific protein binder can act as a potent therapeutic agent for cancers. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Targeted delivery of a human Bcl-2-specific protein binder effectively induces apoptosis of cancer cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000530031800025 | - |
dc.identifier.scopusid | 2-s2.0-85082523018 | - |
dc.type.rims | ART | - |
dc.citation.volume | 526 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 447 | - |
dc.citation.endingpage | 452 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2020.03.113 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Bcl-2 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Protein binder | - |
dc.subject.keywordAuthor | Repebody | - |
dc.subject.keywordAuthor | Cytosolic delivery | - |
dc.subject.keywordAuthor | Cancer cells | - |
dc.subject.keywordPlus | BCL-2 | - |
dc.subject.keywordPlus | REPEBODY | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | BCL-X(L) | - |
dc.subject.keywordPlus | POTENT | - |
dc.subject.keywordPlus | DOMAIN | - |
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