Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics

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dc.contributor.authorLee, Youngseopko
dc.contributor.authorKang, Byoung-Hoonko
dc.contributor.authorKang, Minheeko
dc.contributor.authorChung, Doo Ryeonko
dc.contributor.authorYi, Gwan-Suko
dc.contributor.authorLee, Luke P.ko
dc.contributor.authorJeong, Ki-Hunko
dc.date.accessioned2020-05-08T07:20:12Z-
dc.date.available2020-05-08T07:20:12Z-
dc.date.created2020-05-06-
dc.date.created2020-05-06-
dc.date.created2020-05-06-
dc.date.issued2020-03-
dc.identifier.citationACS APPLIED MATERIALS & INTERFACES, v.12, no.11, pp.12533 - 12540-
dc.identifier.issn1944-8244-
dc.identifier.urihttp://hdl.handle.net/10203/274145-
dc.description.abstractEmerging molecular diagnosis requires ultrafast polymerase chain reaction (PCR) on chip for rapid precise detection of infectious diseases in the point-of-care test. Here, we report nanoplasmonic on-chip PCR for rapid precision molecular diagnostics. The nanoplasmonic pillar arrays (NPA) comprise gold nanoislands on the top and sidewall of large-scale glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light-emitting diode (LED) over the whole visible range due to strong electromagnetic hotspots between the nanoislands. As a result, they effectively induce photothermal heating for ultrafast PCR thermal cycling. The temperature profile of NPA exhibits 30 cycles between 98 and 60 degrees C for a total of 3 min and 30 s during the cyclic excitation of white LED light. The experimental results also demonstrate the rapid DNA amplification of both 0.1 ng mu L-1 of lambda-DNA in 20 thermal cycles and 0.1 ng mu L-1 of complementary DNA of Middle East respiratory syndrome coronavirus in 30 thermal cycles using a conventional PCR volume of 15 mu L. This nanoplasmonic PCR technique provides a new opportunity for rapid precision molecular diagnostics.-
dc.languageEnglish-
dc.publisherAMER CHEMICAL SOC-
dc.titleNanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics-
dc.typeArticle-
dc.identifier.wosid000526543400015-
dc.identifier.scopusid2-s2.0-85081648335-
dc.type.rimsART-
dc.citation.volume12-
dc.citation.issue11-
dc.citation.beginningpage12533-
dc.citation.endingpage12540-
dc.citation.publicationnameACS APPLIED MATERIALS & INTERFACES-
dc.identifier.doi10.1021/acsami.9b23591-
dc.contributor.localauthorYi, Gwan-Su-
dc.contributor.localauthorJeong, Ki-Hun-
dc.contributor.nonIdAuthorKang, Minhee-
dc.contributor.nonIdAuthorChung, Doo Ryeon-
dc.contributor.nonIdAuthorLee, Luke P.-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthormolecular diagnostics-
dc.subject.keywordAuthorpoint-of-care diagnosis-
dc.subject.keywordAuthorpolymerase chain reaction-
dc.subject.keywordAuthornanopillar-
dc.subject.keywordAuthorphotothermal effect-
dc.subject.keywordPlusAMPLIFICATION-
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