DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gurung, Smriti | ko |
dc.contributor.author | Khan, Fatima | ko |
dc.contributor.author | Gunassekaran, Gowri Rangaswamy | ko |
dc.contributor.author | Do Yoo, Jae | ko |
dc.contributor.author | Vadevoo, Sri Murugan Poongkavithai | ko |
dc.contributor.author | Permpoon, Uttapol | ko |
dc.contributor.author | Kim, Sang-Hyun | ko |
dc.contributor.author | Kim, Ha-Jeong | ko |
dc.contributor.author | Kim, In-San | ko |
dc.contributor.author | Han, Hyeonjeong | ko |
dc.contributor.author | Park, Ji-Ho | ko |
dc.contributor.author | Kim, Soyoun | ko |
dc.contributor.author | Lee, Byungheon | ko |
dc.date.accessioned | 2020-05-07T07:20:06Z | - |
dc.date.available | 2020-05-07T07:20:06Z | - |
dc.date.created | 2020-05-06 | - |
dc.date.created | 2020-05-06 | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | BIOMATERIALS, v.247 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://hdl.handle.net/10203/274136 | - |
dc.description.abstract | Blockade of programmed cell death ligand-1 (PD-L1) restores T-cell activity and enhances anti-tumor immunity. Screening a phage-displayed peptide library for peptides that selectively bind to PD-L1-overexpressing cells identified two peptides, CLQKTPKQC and CVRARTR (PD-L1Pep-1 and PD-L1Pep-2, respectively) that appeared to block PD-L1. PD-L1 Pep-1 and PD-L1 Pep-2 preferentially bound to high PD-L1 -expressing cells over low PD-L1-expressing cells; binding was further enhanced by interferon-gamma, an inducer of PD-L1 expression. Binding affinities of PD-L1 Pep-1 and PD-L1 Pep-2 were approximately 373 and 281 nM, respectively. Cellular binding of the PD-L1-binding peptides was reduced by silencing PD-Ll gene expression or competition with anti-PD-Ll antibody. PD-L1 Pep-1 and PD-L1 Pep-2 induced the internalization and downregulated cell surface levels of PD-Ll. The PD-L1-binding peptides restored cytokine secretion and T-cell proliferation to cells inhibited by co-culture with tumor cells or culture on PD-L1-coated plates. Intravenously injected PD-L1 Pep-1 and PD-L1 Pep-2 efficiently homed to tumor tissues, inhibited tumor growth, and increased CD8 + /FoxP3 + ratio in mice. The PD-Ll -binding peptides in combination with doxorubicin or PD-Ll -targeted liposomal doxorubicin inhibited tumor growth and increased CD8 + /FoxP3 + ratio more efficiently than doxorubicin alone and untargeted liposomal doxorubicin, respectively. These results suggest that PD-L1Pep-1 and PD-L1 Pep-2 block PD-Ll and reinvigorate T-cell activity, inhibiting tumor growth by enhancing anti-tumor immunity. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Phage display-identified PD-L1-binding peptides reinvigorate T-cell activity and inhibit tumor progression | - |
dc.type | Article | - |
dc.identifier.wosid | 000526972100004 | - |
dc.identifier.scopusid | 2-s2.0-85082805236 | - |
dc.type.rims | ART | - |
dc.citation.volume | 247 | - |
dc.citation.publicationname | BIOMATERIALS | - |
dc.identifier.doi | 10.1016/j.biomaterials.2020.119984 | - |
dc.contributor.localauthor | Park, Ji-Ho | - |
dc.contributor.nonIdAuthor | Gurung, Smriti | - |
dc.contributor.nonIdAuthor | Khan, Fatima | - |
dc.contributor.nonIdAuthor | Gunassekaran, Gowri Rangaswamy | - |
dc.contributor.nonIdAuthor | Do Yoo, Jae | - |
dc.contributor.nonIdAuthor | Vadevoo, Sri Murugan Poongkavithai | - |
dc.contributor.nonIdAuthor | Permpoon, Uttapol | - |
dc.contributor.nonIdAuthor | Kim, Sang-Hyun | - |
dc.contributor.nonIdAuthor | Kim, Ha-Jeong | - |
dc.contributor.nonIdAuthor | Kim, In-San | - |
dc.contributor.nonIdAuthor | Kim, Soyoun | - |
dc.contributor.nonIdAuthor | Lee, Byungheon | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Immune checkpoint blockade | - |
dc.subject.keywordAuthor | PD-1 | - |
dc.subject.keywordAuthor | PD-L1 | - |
dc.subject.keywordAuthor | Peptides | - |
dc.subject.keywordAuthor | Phage display | - |
dc.subject.keywordAuthor | T-cell activity | - |
dc.subject.keywordPlus | PD-1/PD-L1 INTERACTION | - |
dc.subject.keywordPlus | ANTI-PD-L1 ANTIBODY | - |
dc.subject.keywordPlus | DEATH 1 | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | PD-1 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BLOCKADE | - |
dc.subject.keywordPlus | BLOCKING | - |
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