High-Level Production of Bacteriotoxic Phospholipase Al in Bacterial Host Pseudomonas fluorescens via ABC Transporter-Mediated Secretion and Inducible Expression

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dc.contributor.authorPark, Jiyeonko
dc.contributor.authorEom, Gyeong Taeko
dc.contributor.authorOh, Joon Youngko
dc.contributor.authorPark, Ji Hyunko
dc.contributor.authorKim, Sun Changko
dc.contributor.authorSong, Jae Kwangko
dc.contributor.authorAhn, Jung Hoonko
dc.date.accessioned2020-04-16T09:20:05Z-
dc.date.available2020-04-16T09:20:05Z-
dc.date.created2020-04-14-
dc.date.created2020-04-14-
dc.date.created2020-04-14-
dc.date.created2020-04-14-
dc.date.issued2020-02-
dc.identifier.citationMICROORGANISMS, v.8, no.2-
dc.identifier.issn2076-2607-
dc.identifier.urihttp://hdl.handle.net/10203/273900-
dc.description.abstractBacterial phospholipase A1 (PLA1) is used in various industrial fields because it can catalyze the hydrolysis, esterification, and transesterification of phospholipids to their functional derivatives. It also has a role in the degumming process of crude plant oils. However, bacterial expression of the foreign PLA1-encoding gene was generally hampered because intracellularly expressed PLA1 is inherently toxic and damages the phospholipid membrane. In this study, we report that secretion-based production of recombinant P1aA, a bacterial PLA1 gene, or co-expression of P1aS, an accessory gene, minimizes this harmful effect. We were able to achieve high-level P1aA production via secretion-based protein production. Here, T1iD/T1iE/T1iF, an ABC transporter complex of Pseudomonas fluorescens SIK-W1, was used to secrete recombinant proteins to the extracellular medium. In order to control the protein expression with induction, a new strain of P. fluorescens, which had the lac operon repressor gene lad, was constructed and named ZYAI strain. The bacteriotoxic P1aA protein was successfully produced in a bacterial host, with help from ABC transporter-mediated secretion, induction-controlled protein expression, and fermentation. The final protein product is capable of degumming oil efficiently, signifying its application potential.-
dc.languageEnglish-
dc.publisherMDPI-
dc.titleHigh-Level Production of Bacteriotoxic Phospholipase Al in Bacterial Host Pseudomonas fluorescens via ABC Transporter-Mediated Secretion and Inducible Expression-
dc.typeArticle-
dc.identifier.wosid000519618200095-
dc.identifier.scopusid2-s2.0-85079455569-
dc.type.rimsART-
dc.citation.volume8-
dc.citation.issue2-
dc.citation.publicationnameMICROORGANISMS-
dc.identifier.doi10.3390/microorganisms8020239-
dc.contributor.localauthorKim, Sun Chang-
dc.contributor.nonIdAuthorEom, Gyeong Tae-
dc.contributor.nonIdAuthorOh, Joon Young-
dc.contributor.nonIdAuthorPark, Ji Hyun-
dc.contributor.nonIdAuthorSong, Jae Kwang-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorphospholipase A1 (PLA1)-
dc.subject.keywordAuthorP1aA-
dc.subject.keywordAuthorABC transporter-
dc.subject.keywordAuthorPseudomonas fluorescens-
dc.subject.keywordAuthorprotein secretion-
dc.subject.keywordPlusENZYMATIC DEGUMMING PROCESS-
dc.subject.keywordPlusSERRATIA-LIQUEFACIENS-
dc.subject.keywordPlusPROTEIN-PRODUCTION-
dc.subject.keywordPlusA(1)-
dc.subject.keywordPlusCLONING-
dc.subject.keywordPlusPLASMIDS-
dc.subject.keywordPlusLIPASE-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordPlusENHANCEMENT-
dc.subject.keywordPlusSYSTEMS-
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