DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jiyeon | ko |
dc.contributor.author | Eom, Gyeong Tae | ko |
dc.contributor.author | Oh, Joon Young | ko |
dc.contributor.author | Park, Ji Hyun | ko |
dc.contributor.author | Kim, Sun Chang | ko |
dc.contributor.author | Song, Jae Kwang | ko |
dc.contributor.author | Ahn, Jung Hoon | ko |
dc.date.accessioned | 2020-04-16T09:20:05Z | - |
dc.date.available | 2020-04-16T09:20:05Z | - |
dc.date.created | 2020-04-14 | - |
dc.date.created | 2020-04-14 | - |
dc.date.created | 2020-04-14 | - |
dc.date.created | 2020-04-14 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.citation | MICROORGANISMS, v.8, no.2 | - |
dc.identifier.issn | 2076-2607 | - |
dc.identifier.uri | http://hdl.handle.net/10203/273900 | - |
dc.description.abstract | Bacterial phospholipase A1 (PLA1) is used in various industrial fields because it can catalyze the hydrolysis, esterification, and transesterification of phospholipids to their functional derivatives. It also has a role in the degumming process of crude plant oils. However, bacterial expression of the foreign PLA1-encoding gene was generally hampered because intracellularly expressed PLA1 is inherently toxic and damages the phospholipid membrane. In this study, we report that secretion-based production of recombinant P1aA, a bacterial PLA1 gene, or co-expression of P1aS, an accessory gene, minimizes this harmful effect. We were able to achieve high-level P1aA production via secretion-based protein production. Here, T1iD/T1iE/T1iF, an ABC transporter complex of Pseudomonas fluorescens SIK-W1, was used to secrete recombinant proteins to the extracellular medium. In order to control the protein expression with induction, a new strain of P. fluorescens, which had the lac operon repressor gene lad, was constructed and named ZYAI strain. The bacteriotoxic P1aA protein was successfully produced in a bacterial host, with help from ABC transporter-mediated secretion, induction-controlled protein expression, and fermentation. The final protein product is capable of degumming oil efficiently, signifying its application potential. | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.title | High-Level Production of Bacteriotoxic Phospholipase Al in Bacterial Host Pseudomonas fluorescens via ABC Transporter-Mediated Secretion and Inducible Expression | - |
dc.type | Article | - |
dc.identifier.wosid | 000519618200095 | - |
dc.identifier.scopusid | 2-s2.0-85079455569 | - |
dc.type.rims | ART | - |
dc.citation.volume | 8 | - |
dc.citation.issue | 2 | - |
dc.citation.publicationname | MICROORGANISMS | - |
dc.identifier.doi | 10.3390/microorganisms8020239 | - |
dc.contributor.localauthor | Kim, Sun Chang | - |
dc.contributor.nonIdAuthor | Eom, Gyeong Tae | - |
dc.contributor.nonIdAuthor | Oh, Joon Young | - |
dc.contributor.nonIdAuthor | Park, Ji Hyun | - |
dc.contributor.nonIdAuthor | Song, Jae Kwang | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | phospholipase A1 (PLA1) | - |
dc.subject.keywordAuthor | P1aA | - |
dc.subject.keywordAuthor | ABC transporter | - |
dc.subject.keywordAuthor | Pseudomonas fluorescens | - |
dc.subject.keywordAuthor | protein secretion | - |
dc.subject.keywordPlus | ENZYMATIC DEGUMMING PROCESS | - |
dc.subject.keywordPlus | SERRATIA-LIQUEFACIENS | - |
dc.subject.keywordPlus | PROTEIN-PRODUCTION | - |
dc.subject.keywordPlus | A(1) | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | PLASMIDS | - |
dc.subject.keywordPlus | LIPASE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | SYSTEMS | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.