Femtomolar sensing of Alzheimer's tau proteins by water oxidation-coupled photoelectrochemical platform

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dc.contributor.authorKim, Kayoungko
dc.contributor.authorPark, Chan Beumko
dc.date.accessioned2020-04-09T08:20:09Z-
dc.date.available2020-04-09T08:20:09Z-
dc.date.created2020-04-08-
dc.date.created2020-04-08-
dc.date.issued2020-04-
dc.identifier.citationBIOSENSORS & BIOELECTRONICS, v.154-
dc.identifier.issn0956-5663-
dc.identifier.urihttp://hdl.handle.net/10203/273856-
dc.description.abstractAlzheimer's disease (AD) is the most prevalent neurodegenerative disorder. A key pathogenic event of AD is the formation of intracellular neurofibrillary tangles that are mainly composed of tau proteins. Here, we report on ultrasensitive detection of total tau (t-tau) proteins using an artificial electron donor-free, BiVO4-based photoelectrochemical (PEC) analysis. The platform was constructed by incorporating molybdenum (Mo) dopant and iron oxyhydroxide (FeOOH) ad-layer into the BiVO4 photoelectrode and employing a signal amplifier formed by horseradish peroxidase (HRP)-triggered oxidation of 3,3'-diaminobenzidine (DAB). Despite the absence of additional electron suppliers, the FeOOH/Mo:BiVO4 conjugated with the Tau5 antibody produced strong current signals at 0 V (vs. Ag/AgCl, 3 M NaCl) under the illumination of a white light-emitting diode. The Mo extrinsic dopants increased the charge carrier density of BiVO4-Tau5 by 1.57 times, and the FeOOH co-catalyst promoted the interfacial water oxidation reaction of Mo:BiVO4-Tau5 by suppressing charge recombination. The introduction of HRP-labeled Tau46 capture antibodies to the FeOOH/Mo:BiVO4-Tau5 platform produced insoluble precipitation on the transducer by accelerating the oxidation of DAB, which amplified the photocurrent signal of FeOOH/Mo:BiVO4-Tau5 by 2.07-fold. Consequently, the water oxidation-coupled, FeOOH/Mo:BiVO4-based PEC sensing platform accurately and selectively recognized t-tau proteins down to femtomolar concentrations; the limit of detection and limit of quantification were determined to be 1.59 fM and 4.11 fM, respectively.-
dc.languageEnglish-
dc.publisherELSEVIER ADVANCED TECHNOLOGY-
dc.titleFemtomolar sensing of Alzheimer's tau proteins by water oxidation-coupled photoelectrochemical platform-
dc.typeArticle-
dc.identifier.wosid000519667000019-
dc.identifier.scopusid2-s2.0-85079224248-
dc.type.rimsART-
dc.citation.volume154-
dc.citation.publicationnameBIOSENSORS & BIOELECTRONICS-
dc.identifier.doi10.1016/j.bios.2020.112075-
dc.contributor.localauthorPark, Chan Beum-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorAlzheimer&apos-
dc.subject.keywordAuthors disease-
dc.subject.keywordAuthorTau proteins-
dc.subject.keywordAuthorFemtomolar sensitivity-
dc.subject.keywordAuthorBiVO4-
dc.subject.keywordAuthorWater oxidation-
dc.subject.keywordPlusAMYLOID-BETA-
dc.subject.keywordPlusBIVO4 PHOTOANODES-
dc.subject.keywordPlusELECTROCHEMICAL IMMUNOSENSOR-
dc.subject.keywordPlusCOLORIMETRIC DETECTION-
dc.subject.keywordPlusDISEASE-
dc.subject.keywordPlusBIOSENSOR-
dc.subject.keywordPlusDIAGNOSIS-
dc.subject.keywordPlusBIOMARKERS-
dc.subject.keywordPlusEFFICIENT-
dc.subject.keywordPlusEVOLUTION-
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