DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Kayoung | ko |
dc.contributor.author | Park, Chan Beum | ko |
dc.date.accessioned | 2020-04-09T08:20:09Z | - |
dc.date.available | 2020-04-09T08:20:09Z | - |
dc.date.created | 2020-04-08 | - |
dc.date.created | 2020-04-08 | - |
dc.date.created | 2020-04-08 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.154 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/273856 | - |
dc.description.abstract | Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder. A key pathogenic event of AD is the formation of intracellular neurofibrillary tangles that are mainly composed of tau proteins. Here, we report on ultrasensitive detection of total tau (t-tau) proteins using an artificial electron donor-free, BiVO4-based photoelectrochemical (PEC) analysis. The platform was constructed by incorporating molybdenum (Mo) dopant and iron oxyhydroxide (FeOOH) ad-layer into the BiVO4 photoelectrode and employing a signal amplifier formed by horseradish peroxidase (HRP)-triggered oxidation of 3,3'-diaminobenzidine (DAB). Despite the absence of additional electron suppliers, the FeOOH/Mo:BiVO4 conjugated with the Tau5 antibody produced strong current signals at 0 V (vs. Ag/AgCl, 3 M NaCl) under the illumination of a white light-emitting diode. The Mo extrinsic dopants increased the charge carrier density of BiVO4-Tau5 by 1.57 times, and the FeOOH co-catalyst promoted the interfacial water oxidation reaction of Mo:BiVO4-Tau5 by suppressing charge recombination. The introduction of HRP-labeled Tau46 capture antibodies to the FeOOH/Mo:BiVO4-Tau5 platform produced insoluble precipitation on the transducer by accelerating the oxidation of DAB, which amplified the photocurrent signal of FeOOH/Mo:BiVO4-Tau5 by 2.07-fold. Consequently, the water oxidation-coupled, FeOOH/Mo:BiVO4-based PEC sensing platform accurately and selectively recognized t-tau proteins down to femtomolar concentrations; the limit of detection and limit of quantification were determined to be 1.59 fM and 4.11 fM, respectively. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Femtomolar sensing of Alzheimer's tau proteins by water oxidation-coupled photoelectrochemical platform | - |
dc.type | Article | - |
dc.identifier.wosid | 000519667000019 | - |
dc.identifier.scopusid | 2-s2.0-85079224248 | - |
dc.type.rims | ART | - |
dc.citation.volume | 154 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2020.112075 | - |
dc.contributor.localauthor | Park, Chan Beum | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Alzheimer&apos | - |
dc.subject.keywordAuthor | s disease | - |
dc.subject.keywordAuthor | Tau proteins | - |
dc.subject.keywordAuthor | Femtomolar sensitivity | - |
dc.subject.keywordAuthor | BiVO4 | - |
dc.subject.keywordAuthor | Water oxidation | - |
dc.subject.keywordPlus | AMYLOID-BETA | - |
dc.subject.keywordPlus | BIVO4 PHOTOANODES | - |
dc.subject.keywordPlus | ELECTROCHEMICAL IMMUNOSENSOR | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | EFFICIENT | - |
dc.subject.keywordPlus | EVOLUTION | - |
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